Yameogo Noé, Bazie Bapio Valérie Elvira Jean Télesphore, Ouattara Abdoul Karim, Yameogo Pouiré, Compaore Tegwinde Rebeca, Obiri-Yeboah Dorcas, Djigma Florencia Wenkuuni, Karou Simplice Damintoti, Simpore Jacques
Laboratoire de Biologie Moléculaires et de Génétique (LABIOGENE), UFR-SVT, Université de Ouaga I Professeur Joseph KI-ZERBO, Ouagadougou, Burkina Faso.
Département Substances Naturelles (DSN), Institut de Recherche en Sciences Appliquées et Technologies (IRSAT), Ouagadougou, Burkina Faso.
Malar Res Treat. 2017;2017:3468276. doi: 10.1155/2017/3468276. Epub 2017 May 18.
This study analyzed the four main polymorphisms of the genes in homocysteine metabolism in malaria patients. Forty-two randomly selected subjects, diagnosed positive for were included. The four genotypes were detected by real-time PCR using the MTHFR 677C>T, MTHFR 1298A>C, MTR 2756A>G, and MTRR 66A>G detection kit (Sacace Biotechnologies REF: T01002-96-S). The results revealed frequencies of 90% 677CC, 10% 677CT, and 00% 677TT for MTHFR C677T; 78.6% 1298AA, 19% 1298AC, and 2.4% 1298CC for MTHFR A1298C; 61.9% 2756AA, 33.3% 2756AG, and 4.8% 2756GG for MTR A2756G; and 50% of 66AA, 45% of 66AG, and 5% of 66GG for MTRR A66G. Correlations were found between A2756G MTR genotypes and parasitaemia ( = 0.02), MTRR A66G and hemoglobin genotypes ( = 0.009), and MTHFR A1298C and sex ( = 0.01). This study demonstrated for the first time an association between the A2756G MTR alleles and malaria in Burkina Faso and gave an overview of the genotypic distribution of the major SNPs influencing the metabolism of homocysteine.
本研究分析了疟疾患者同型半胱氨酸代谢相关基因的四种主要多态性。纳入了42名随机选择的、被诊断为阳性的受试者。使用MTHFR 677C>T、MTHFR 1298A>C、MTR 2756A>G和MTRR 66A>G检测试剂盒（Sacace生物技术公司编号：T01002-96-S）通过实时PCR检测四种基因型。结果显示，MTHFR C677T的基因型频率为90% 677CC、10% 677CT和0% 677TT；MTHFR A1298C的基因型频率为78.6% 1298AA、19% 1298AC和2.4% 1298CC；MTR A2756G的基因型频率为61.9% 2756AA、33.3% 2756AG和4.8% 2756GG；MTRR A66G的基因型频率为50% 66AA、45% 66AG和5% 66GG。发现MTR A2756G基因型与疟原虫血症之间存在相关性（P = 0.02），MTRR A66G与血红蛋白基因型之间存在相关性（P = 0.009），MTHFR A1298C与性别之间存在相关性（P = 0.01）。本研究首次证明了布基纳法索MTR A2756G等位基因与疟疾之间的关联，并概述了影响同型半胱氨酸代谢的主要单核苷酸多态性的基因型分布。
