Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, Hubei 430062, P.R. China.
Analyst. 2017 Jul 10;142(14):2617-2623. doi: 10.1039/c7an00553a.
Graphitic carbon nitride (g-CN) nanosheets are a type of two-dimensional nanomaterial with stable photoluminescence and large specific surface areas that make them an ideal candidate for fluorescence sensing. However, fluorescence sensing based on g-CN nanosheets for disease-specific biomolecules is still limited. Moreover, current g-CN nanosheet-based fluorescence sensors for nucleic acid require pre-labeling which is time-consuming and cost-ineffective. In this work, a novel label-free strategy for HBV gene determination based on metal ion binding-induced fluorescence quenching of g-CN nanosheets was designed. Under the optimized conditions, this bioassay provided high sensitivity for HBV gene determination with the limit of detection as low as 1.0 nM, and has been successfully applied for the detection of HBV gene in human serum with satisfactory results. By embedding different recognition sequences of the probes, this method may be conveniently used for versatile detection of diverse disease markers. This novel design may provide a simple, flexible, sensitive and low-cost strategy for g-CN nanosheet-based disease-specific biomolecule sensing.
石墨相氮化碳（g-CN）纳米片是一种具有稳定光致发光和大比表面积的二维纳米材料，使其成为荧光传感的理想候选材料。然而，基于 g-CN 纳米片的用于疾病特异性生物分子的荧光传感仍然受到限制。此外，目前基于 g-CN 纳米片的用于核酸的荧光传感器需要预标记，这既耗时又不经济。在这项工作中，设计了一种基于金属离子结合诱导 g-CN 纳米片荧光猝灭的用于 HBV 基因测定的新型无标记策略。在优化条件下，该生物测定法为 HBV 基因测定提供了高灵敏度，检测限低至 1.0 nM，并已成功应用于人血清中 HBV 基因的检测，结果令人满意。通过嵌入探针的不同识别序列，该方法可方便地用于多种疾病标志物的检测。这种新设计可能为基于 g-CN 纳米片的疾病特异性生物分子传感提供一种简单、灵活、灵敏且低成本的策略。
