基于石墨相氮化碳纳米片作为纳米荧光探针的纳摩尔级二甲双胍灵敏荧光传感策略。

A sensitive fluorescent sensing strategy for nanomolar levels of metformin using graphitic carbon nitride nanosheets as nanofluoroprobe.

机构信息

Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Medicinal Chemistry Departments, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

出版信息

Anal Chim Acta. 2018 Oct 5;1026:117-124. doi: 10.1016/j.aca.2018.04.007. Epub 2018 Apr 17.

DOI:10.1016/j.aca.2018.04.007

PMID:29852987

Abstract

A simple and green fluorescence detection system on the basis of the quenching effect of Cu(II) ions on the graphitic carbon nitride nanosheets (g-CN NS) fluorescence and recording the changes of restored fluorescence intensity upon the addition of metformin (MET) was developed for the first time. The g-CN NS were produced using high-temperature polymerization of melamine followed by ultrasonication-assisted liquid exfoliation. The method exhibited excellent sensitivity and accuracy toward MET with the detection as low as 3 nM and linear calibration curve in the range of 0.01-20 μM. In addition, this assay was successfully applied to the determination of MET concentration in human serum samples. It is robust and is suitable for fast, sensitive and cost effective measurement of MET in biological materials.

摘要

首次开发了一种基于 Cu(II) 离子对石墨相氮化碳纳米片（g-CN NS）荧光的淬灭效应及记录加入二甲双胍（MET）后恢复荧光强度变化的简单绿色荧光检测系统。g-CN NS 是通过三聚氰胺的高温聚合，然后进行超声辅助的液相剥离制备而成。该方法对 MET 具有优异的灵敏度和准确性，检测限低至 3 nM，在 0.01-20 μM 范围内呈线性校准曲线。此外，该测定法成功应用于人血清样品中 MET 浓度的测定。该方法稳定，适用于生物材料中 MET 的快速、灵敏和经济有效的测量。