Ocheja Ohiemi Benjamin, Ayo Joseph Olusegun, Aluwong Tagang, Minka Ndazo Salka
Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, PMB 1045, Nigeria.
Department of Veterinary Physiology, Faculty of Veterinary Medicine, University of Benin, Benin City, Nigeria.
Trop Anim Health Prod. 2017 Aug;49(6):1273-1280. doi: 10.1007/s11250-017-1325-5. Epub 2017 Jun 12.
The experiment investigated the ameliorative effects of L-glutamine administration on rectal temperature (RT), erythrocyte osmotic fragility (EOF), serum antioxidant enzyme activities and malondialdehyde (MDA) concentration in Red Sokoto goats during the hot-dry season. Twenty eight healthy Red Sokoto goats, comprising 14 experimental (administered 0.2 g/kg of L-glutamine dissolved in 10 mL of distilled water, once daily for 21 days) and 14 control (administered equivalent of distilled water) goats served as subjects. Rectal temperature (measured at 6:00, 13:00 and 18:00 h) and blood samples (taken at 8:00 h) were obtained from all subjects weekly, before, during and after L-glutamine administration. Data obtained were compared using one-way repeated-measures ANOVA, followed by Tukey's post-hoc test. The dry-bulb temperature, relative humidity and temperature-humidity index for the study period ranged between 24.0 and 37.5 °C, 26.0 and 84.0% and 73.0 and 86.3, respectively. L-glutamine administration decreased (P < 0.05) RT, EOF and MDA and increased superoxide dismutase (SOD) activity in experimental group, compared to controls during weeks 1, 2 and 3. Glutathione peroxidase (GPx) and catalase activities were higher (P < 0.05) in the experimental group than in the controls only during week 1 of L-glutamine administration. In conclusion, L-glutamine administration mitigated increases in RT, EOF and serum MDA concentration and enhanced serum SOD, GPx and catalase activities and may be beneficial in heat-stressed goats during the hot-dry season.
该实验研究了在炎热干燥季节给红索科托山羊施用L-谷氨酰胺对直肠温度（RT）、红细胞渗透脆性（EOF）、血清抗氧化酶活性和丙二醛（MDA）浓度的改善作用。28只健康的红索科托山羊，其中14只为实验组（每天一次，连续21天，每只羊经口灌服溶解于10 mL蒸馏水中的0.2 g/kg L-谷氨酰胺），14只为对照组（灌服等量蒸馏水）作为实验对象。在施用L-谷氨酰胺前、施用期间和施用后，每周从所有实验对象采集直肠温度（分别于6:00、13:00和18:00测量）和血样（于8:00采集）。所获数据采用单因素重复测量方差分析进行比较，随后进行Tukey事后检验。研究期间的干球温度、相对湿度和温湿度指数分别在24.0至37.5℃、26.0至84.0%和73.0至86.3之间。与对照组相比，在第1、2和3周，实验组施用L-谷氨酰胺后直肠温度、红细胞渗透脆性和丙二醛降低（P<0.05），超氧化物歧化酶（SOD）活性升高。仅在施用L-谷氨酰胺的第1周，实验组的谷胱甘肽过氧化物酶（GPx）和过氧化氢酶活性高于对照组（P<0.05）。总之，施用L-谷氨酰胺可缓解直肠温度、红细胞渗透脆性和血清丙二醛浓度的升高，并增强血清SOD、GPx和过氧化氢酶活性，可能对炎热干燥季节热应激山羊有益。
