Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, PMB 1045, Zaria, Nigeria.
Department of Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, PMB 1045, Zaria, Nigeria.
J Therm Biol. 2020 May;90:102571. doi: 10.1016/j.jtherbio.2020.102571. Epub 2020 Mar 19.
The aim of the study was to evaluate haematological responses in Red Sokoto goats (RSGs) administered with L-glutamine during the hot-dry season. Experimental animals included 28 clinically healthy RSGs divided into treated group (n = 14); each administered L-glutamine at 0.2 g/kg body weight, dissolved in 10 mL distilled water, and control group (n = 14); each administered 10 mL distilled water, per os once daily for 21 days. The ambient temperature and relative humidity recorded daily for 4 weeks were used to calculate the temperature-humidity index. Three millilitres of blood sample was collected from each goat by jugular venipuncture for haematology, while rectal temperature (RT), heart rate (HR) and respiratory rate (RR) were also measured once weekly at weeks 0 (before), 1, 2, 3 (during) and 4 (after L-glutamine administration). The haematological, RT, HR and RR data obtained weekly were analysed using repeated-measures one-way ANOVA, followed by Tukey's post-hoc test to evaluate differences between periods, and between treated and control groups. The PCV, haemoglobin concentration and RBC count were higher (P < 0.05) in the treated group compared to the control group during the period of L-glutamine administration. These differences were sustained till week 4. Beginning from week 1 of the study, the total leucocyte count in treated group (10.10 ± 0.25 × 10/μL) was higher (P < 0.05) than the count in control group (7.23 ± 0.41 × 10/μL), this trend was also maintained throughout the study. The neutrophil:lymphocyte ratio during weeks 3 and 4 of the experiment was lower (P < 0.05) in the treated compared to the control group. RT was lower (P < 0.05) in treated group than the control group. In conclusion, L-glutamine administration ameliorated the adverse effects of heat stress on the haematological parameters in RSGs during the hot-dry season.
本研究旨在评估在炎热干燥季节给 Red Sokoto 山羊（RSG）施用 L-谷氨酰胺后的血液学反应。实验动物包括 28 只临床健康的 RSG，分为治疗组（n=14）；每只动物给予 0.2 g/kg 体重的 L-谷氨酰胺，溶解在 10 mL 蒸馏水中，和对照组（n=14）；每只动物给予 10 mL 蒸馏水，每天口服一次，共 21 天。在四周内，每天记录环境温度和相对湿度，以计算温度-湿度指数。通过颈静脉穿刺从每只山羊采集 3 毫升血液样本进行血液学检查，同时每周一次测量直肠温度（RT）、心率（HR）和呼吸频率（RR），分别在第 0 周（给药前）、1 周、2 周、3 周（给药期间）和 4 周（给药后）进行。每周获得的血液学、RT、HR 和 RR 数据采用重复测量的单向方差分析进行分析，然后采用 Tukey 的事后检验来评估不同时期以及治疗组和对照组之间的差异。在给予 L-谷氨酰胺期间，治疗组的 PCV、血红蛋白浓度和 RBC 计数均高于对照组（P<0.05）。这些差异一直持续到第 4 周。从研究的第 1 周开始，治疗组的总白细胞计数（10.10±0.25×10/μL）高于对照组（7.23±0.41×10/μL）（P<0.05），这种趋势在整个研究过程中都得到了维持。在实验的第 3 和第 4 周，治疗组的中性粒细胞与淋巴细胞的比值低于对照组（P<0.05）。与对照组相比，治疗组的 RT 较低（P<0.05）。总之，在炎热干燥季节，L-谷氨酰胺的给药改善了热应激对 RSG 血液学参数的不利影响。
