拟南芥AGO蛋白在减数分裂和DNA修复中的功能分析

Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair.

作者信息

Martinez-Garcia Marina, Pradillo Mónica

机构信息

School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK.

Departamento de Genética, Facultad de Biología, Universidad Complutense de Madrid, C/Jose Antonio Novais 12, Madrid, 28040, Spain.

出版信息

Methods Mol Biol. 2017;1640:145-158. doi: 10.1007/978-1-4939-7165-7_10.

DOI:10.1007/978-1-4939-7165-7_10

PMID:28608340

Abstract

Plant ARGONAUTE (AGO) proteins regulate a wide range of cellular and developmental functions. Recent findings highlight their role during homologous recombination, a basic mechanism to repair double-strand DNA lesions (in somatic cells) and programmed DNA breaks (in meiocytes). This chapter contains an exhaustive description of procedures applied to analyze meiotic chromosome behavior (cytogenetic techniques) and DNA repair capacity (genotoxicity assays) in AGO-deficient Arabidopsis thaliana mutants.

摘要

植物中的AGO蛋白调控多种细胞和发育功能。最近的研究结果突出了它们在同源重组过程中的作用，同源重组是一种修复双链DNA损伤（在体细胞中）和程序性DNA断裂（在减数分裂细胞中）的基本机制。本章详细描述了用于分析AGO缺陷型拟南芥突变体减数分裂染色体行为（细胞遗传学技术）和DNA修复能力（遗传毒性测定）的方法。