Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, USA.
Lab Chip. 2017 Jun 27;17(13):2294-2302. doi: 10.1039/c7lc00412e.
Here we demonstrate that microfluidic cell culture devices, known as Organs-on-a-Chips can be fabricated with multifunctional, real-time, sensing capabilities by integrating both multi-electrode arrays (MEAs) and electrodes for transepithelial electrical resistance (TEER) measurements into the chips during their fabrication. To prove proof-of-concept, simultaneous measurements of cellular electrical activity and tissue barrier function were carried out in a dual channel, endothelialized, heart-on-a-chip device containing human cardiomyocytes and a channel-separating porous membrane covered with a primary human endothelial cell monolayer. These studies confirmed that the TEER-MEA chip can be used to simultaneously detect dynamic alterations of vascular permeability and cardiac function in the same chip when challenged with the inflammatory stimulus tumor necrosis factor alpha (TNF-α) or the cardiac targeting drug isoproterenol. Thus, this Organ Chip with integrated sensing capability may prove useful for real-time assessment of biological functions, as well as response to therapeutics.
在这里，我们证明了微流控细胞培养设备（即芯片上器官）可以通过在制造过程中将多电极阵列 (MEA) 和用于跨上皮电阻 (TEER) 测量的电极集成到芯片中，从而具有多功能、实时传感功能。为了验证概念验证，在包含人心肌细胞和覆盖有原代人内皮细胞单层的多孔膜的通道分离多孔膜的双通道内皮化心脏芯片设备中进行了细胞电活动和组织屏障功能的同时测量。这些研究证实，当受到炎症刺激肿瘤坏死因子 α (TNF-α) 或心脏靶向药物异丙肾上腺素的挑战时，TEER-MEA 芯片可用于同时检测同一芯片中血管通透性和心脏功能的动态变化。因此，这种具有集成传感功能的器官芯片可能有助于实时评估生物学功能以及对治疗的反应。
