Williams G D, Holt S C
Can J Microbiol. 1985 Mar;31(3):238-50. doi: 10.1139/m85-046.
The outer membranes from selected oral Bacteroides species were isolated and characterized morphologically, chemically, and physically. Both sucrose density gradient centrifugation and sodium dodecyl sulfate--polyacrylamide gel electrophoresis (SDS-PAGE) revealed outer membranes which varied slightly with the species, as well as showing complex polypeptide patterns after SDS--PAGE. The polypeptide distribution revealed a species-specific pattern; however, there was often a variation within a given species in many minor polypeptide bands, with heat-modifiable minor peptides occurring in nearly all species. Protein-associated carbohydrates occurred in several species. Outer membrane fragments (blebs) recovered by ultracentrifugation of the growth supernatants were either nearly identical to the outer membrane polypeptide patterns, or contained reduced amounts of specific polypeptides, again varying with the species. 125I-labelling of whole cells indicated possible surface exposure for several of the major polypeptides.
从选定的口腔拟杆菌属物种中分离出外膜，并对其进行形态学、化学和物理特性分析。蔗糖密度梯度离心和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）均显示，外膜随物种不同略有差异，且在SDS-PAGE后呈现出复杂的多肽图谱。多肽分布呈现物种特异性模式；然而，在许多次要多肽条带中，给定物种内通常存在差异，几乎所有物种中都存在热可修饰的次要肽。几种物种中存在与蛋白质相关的碳水化合物。通过超速离心生长上清液回收的外膜片段（泡）要么与外膜多肽图谱几乎相同，要么含有特定多肽的减少量，同样因物种而异。全细胞的125I标记表明，几种主要多肽可能暴露于表面。
