Naito Y, Okuda K, Kato T, Takazoe I
Infect Immun. 1985 Oct;50(1):231-5. doi: 10.1128/iai.50.1.231-235.1985.
Monoclonal antibodies against the various surface antigens of Bacteroides gingivalis were obtained by the fusion of murine myeloma cells (SP2/0-Ag14) with spleen cells of BALB/c mice immunized with the whole cells. Two monoclonal antibodies reacted with lipopolysaccharide, and the other two reacted strongly with capsule antigen. One showed reactivity with the hemagglutinin of the cells. The five monoclonal antibodies reacted with sonicated antigen from all B. gingivalis strains tested. No cross-reactivity of the monoclonal antibodies with antigens from nine species of other black-pigmented Bacteroids strains was observed. An immunoblotting test involving the use of these monoclonal antibodies indicated that the epitope of B. gingivalis lipopolysaccharide was polysaccharide with a high molecular weight of 40,000 to 60,000. The immunoblotting test also demonstrated that the epitopes of capsule antigen and of hemagglutinin were 27,000- and 40,000-molecular-weight proteins, respectively.
通过将鼠骨髓瘤细胞（SP2/0-Ag14）与用全细胞免疫的BALB/c小鼠的脾细胞融合，获得了针对牙龈拟杆菌各种表面抗原的单克隆抗体。两种单克隆抗体与脂多糖反应，另外两种与荚膜抗原强烈反应。一种与细胞的血凝素反应。这五种单克隆抗体与所有测试的牙龈拟杆菌菌株的超声处理抗原反应。未观察到单克隆抗体与其他九种黑色色素拟杆菌菌株的抗原发生交叉反应。涉及使用这些单克隆抗体的免疫印迹试验表明，牙龈拟杆菌脂多糖的表位是分子量为40,000至60,000的高分子量多糖。免疫印迹试验还表明，荚膜抗原和血凝素的表位分别是分子量为27,000和40,000的蛋白质。
