Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, No. 27 Shanda South Road, Jinan, 250100 Shandong, PR China.
Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, No. 27 Shanda South Road, Jinan, 250100 Shandong, PR China.
Bioresour Technol. 2017 Oct;241:681-691. doi: 10.1016/j.biortech.2017.05.203. Epub 2017 Jun 1.
A permanent microbial stratified nitrogen removal system coupling anammox with partial nitrification (SNAP) in a sequencing batch biofilter granular reactor (SBBGR) was successfully constructed for the treatment of ammonia-rich wastewater. With a nitrogen loading rate of 0.1kgNm·d, the maximal ammonia and total nitrogen removal efficiencies could reach up to 96.08% and 84.86% on day 108, respectively. The pH, DO profiles revealed a switch of functional species (AOB and anammox) at a typical intermittent aeration cycle. qPCR and high throughput analyses certified a stable spatial microbial stratified community structure. Although, anammox preferred strict anaerobic environment while AOB needed oxygen, a special stratified community structure contributed to conquer this obstacle. Moreover, Bacteroidet, Chlorobi, OD1, Planctomycetes, and Proteobacteria were the dominant species in the SBBGR. Although we have predicted the possible pathways of nitrogen transformation, further studies are needed to validate the pathways in enzymology.
采用序批式生物滤池颗粒反应器（SBBGR）成功构建了一种将厌氧氨氧化与部分硝化（SNAP）偶联的永久微生物分层脱氮系统，用于处理富氨废水。在氮负荷为 0.1kgNm·d 的情况下，第 108 天氨氮和总氮的最大去除效率分别高达 96.08%和 84.86%。pH、DO 分布揭示了在典型的间歇曝气周期中功能物种（AOB 和厌氧氨氧化菌）的转换。qPCR 和高通量分析证实了稳定的空间微生物分层群落结构。尽管厌氧氨氧化菌更喜欢严格的厌氧环境，而 AOB 需要氧气，但特殊的分层群落结构有助于克服这一障碍。此外，拟杆菌门、绿弯菌门、OD1 门、浮霉菌门和变形菌门是 SBBGR 中的优势物种。尽管我们已经预测了氮转化的可能途径，但仍需要进一步的研究来验证酶学中的途径。
