Hilmy A M, Shabana M B, Daabees A Y
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;81(1):145-53. doi: 10.1016/0742-8413(85)90106-9.
This study reports changes in total protein and certain liver, heart, gill and serum enzymes after exposing fish or tissue homogenates and serum to Cd, i.e. in vivo and in vitro effects. Five enzymes were selected for assay; aspartate amino transferase (AAT), alanine amino transferase (A1AT), alkaline phosphatase (A1P), acid phosphatase (ACP) and lactic dehydrogenase (LDH). Total protein content in the exposed fish showed an increase in the liver, gill and serum while there was no change in heart protein. The sensitivity of the five assayed enzymes to Cd varied in different tissues. Gill AAT, A1AT were the most sensitive. Liver A1P and heart LDH showed the maximum inhibition at higher Cd concentrations.
本研究报告了将鱼类、组织匀浆和血清暴露于镉后，即体内和体外效应下，总蛋白以及某些肝脏、心脏、鳃和血清酶的变化。选择了五种酶进行测定：天冬氨酸氨基转移酶（AAT）、丙氨酸氨基转移酶（A1AT）、碱性磷酸酶（A1P）、酸性磷酸酶（ACP）和乳酸脱氢酶（LDH）。暴露于镉的鱼的肝脏、鳃和血清中的总蛋白含量增加，而心脏蛋白没有变化。所测定的五种酶对镉的敏感性在不同组织中有所不同。鳃中的AAT、A1AT最为敏感。肝脏中的A1P和心脏中的LDH在较高镉浓度下表现出最大抑制作用。
