Mann Marcus, Serif Manuel, Jakob Torsten, Kroth Peter G, Wilhelm Christian
Institute of Biology, Department of Plant Physiology, University of Leipzig, D-04103 Leipzig, Germany.
Plant Ecophysiology, Fachbereich Biologie, Universität Konstanz, D-78457 Konstanz, Germany.
J Plant Physiol. 2017 Oct;217:44-48. doi: 10.1016/j.jplph.2017.05.020. Epub 2017 May 31.
Aureochromes are blue light receptors specifically found in photosynthetic Stramenopiles (algae). Four different Aureochromes have been identified in the marine diatom Phaeodactylum tricornutum (PtAUREO 1a, 1b, 1c, and 2). Since blue light is necessary for high light acclimation in diatoms, it has been hypothesized that Aureochromes might play an important role in the light acclimation capacity of diatoms. This hypothesis was supported by an RNAi knockdown line of PtAUREO1a, which showed a phenotype different from wild type cells when grown in either blue or red light. Here, we show for the first time the phenotype and the photoacclimation reaction of TALEN-mediated knockout mutants of PtAUREO1a and PtAUREO1b, clearly proving the necessity of Aureochromes for light acclimation under blue light. However, both mutants do also show specific differences in their respective phenotypes. Hence, PtAUREO1a and 1b are not functionally redundant in photoacclimation to blue light, and their specific contribution needs to be clarified further.
藻红素是光合不等鞭毛类（藻类）中特有的蓝光受体。在海洋硅藻三角褐指藻（Phaeodactylum tricornutum，PtAUREO 1a、1b、1c和2）中已鉴定出四种不同的藻红素。由于蓝光对于硅藻的高光适应是必需的，因此有人推测藻红素可能在硅藻的光适应能力中发挥重要作用。这一假设得到了PtAUREO1a的RNA干扰敲除株系的支持，该株系在蓝光或红光下生长时表现出与野生型细胞不同的表型。在这里，我们首次展示了TALEN介导的PtAUREO1a和PtAUREO1b基因敲除突变体的表型和光适应反应，明确证明了藻红素在蓝光下光适应中的必要性。然而，这两种突变体在各自的表型上也确实存在特定差异。因此，PtAUREO1a和1b在蓝光光适应中功能并非冗余，它们的具体作用还需要进一步阐明。
