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Pt3菌株梭形、椭圆形和三辐射形态型的比较结构与功能分析

Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes of Pt3 Strain.

作者信息

Galas Ludovic, Burel Carole, Schapman Damien, Ropitaux Marc, Bernard Sophie, Bénard Magalie, Bardor Muriel

机构信息

Normandie University, UNIROUEN, INSERM, PRIMACEN, Rouen, France.

Normandie University, UNIROUEN, Laboratoire Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV) EA4358, Rouen, France.

出版信息

Front Plant Sci. 2021 Apr 12;12:638181. doi: 10.3389/fpls.2021.638181. eCollection 2021.

DOI:10.3389/fpls.2021.638181
PMID:33912207
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8072121/
Abstract

The diatom is a marine unicellular microalga that exists under three main morphotypes: oval, fusiform, and triradiate. Previous works have demonstrated that the oval morphotype of Pt3 strain presents specific metabolic features. Here, we compared the cellular organization of the main morphotypes of the diatom Pt3 strain through transmission electron and advanced light microscopies. The three morphotypes share similarities including spectral characteristics of the plastid, the location of the nucleus, the organization of mitochondria around the plastid as well as the existence of both a F-actin cortex, and an intracellular network of F-actin. In contrast, compared to fusiform and triradiate cells, oval cells spontaneously release proteins more rapidly. In addition, comparison of whole transcriptomes of oval versus fusiform or triradiate cells revealed numerous differential expression of positive and negative regulators belonging to the complex dynamic secretory machinery. This study highlights the specificities occurring within the oval morphotype underlying that the oval cells secrete proteins more rapidly.

摘要

硅藻是一种海洋单细胞微藻，存在三种主要形态类型：椭圆形、梭形和三辐射形。先前的研究表明，Pt3菌株的椭圆形形态具有特定的代谢特征。在此，我们通过透射电子显微镜和先进的光学显微镜比较了硅藻Pt3菌株主要形态类型的细胞结构。这三种形态类型具有相似之处，包括质体的光谱特征、细胞核的位置、质体周围线粒体的组织方式以及F-肌动蛋白皮层和细胞内F-肌动蛋白网络的存在。相比之下，与梭形和三辐射形细胞相比，椭圆形细胞自发释放蛋白质的速度更快。此外，对椭圆形细胞与梭形或三辐射形细胞的全转录组进行比较，发现属于复杂动态分泌机制的正负调节因子存在大量差异表达。这项研究突出了椭圆形形态类型中存在的特异性，即椭圆形细胞分泌蛋白质的速度更快。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/8fb4fc7aa403/fpls-12-638181-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/715505e42683/fpls-12-638181-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/51745f35a98d/fpls-12-638181-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/6e88b2be475c/fpls-12-638181-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/d5a7ef6b37f4/fpls-12-638181-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/bb2caa6381f0/fpls-12-638181-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/795b153a635d/fpls-12-638181-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/8fb4fc7aa403/fpls-12-638181-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/715505e42683/fpls-12-638181-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/85ae3d8d133e/fpls-12-638181-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/51745f35a98d/fpls-12-638181-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/6e88b2be475c/fpls-12-638181-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/d5a7ef6b37f4/fpls-12-638181-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/bb2caa6381f0/fpls-12-638181-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/795b153a635d/fpls-12-638181-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b0/8072121/8fb4fc7aa403/fpls-12-638181-g008.jpg

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