Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
Biological Germplasm Resources Identification Center of AQSIQ, Beijing, 100176, China.
Sci Rep. 2017 Jun 13;7(1):3427. doi: 10.1038/s41598-017-02083-7.
Paris is famous in China for its medicinal value and has been included in the Chinese Pharmacopoeia. Inaccurate identification of these species could confound their effective exploration, conservation, and domestication. Due to the plasticity of the morphological characteristics, correct identification among Paris species remains problematic. In this regard, we report the complete chloroplast genome of P. thibetica and P. rugosa to develop highly variable molecular markers. Comparing three chloroplast genomes, we sought out the most variable regions to develop the best cpDNA barcodes for Paris. The size of Paris chloroplast genome ranged from 162,708 to 163,200 bp. A total of 134 genes comprising 81 protein coding genes, 45 tRNA genes and 8 rRNA genes were observed in all three chloroplast genomes. Eight rapidly evolving regions were detected, as well as the difference of simple sequence repeats (SSR) and repeat sequence. Two regions of the coding gene ycf1, ycf1a and ycf1b, evolved the quickest and were proposed as core barcodes for Paris. The complete chloroplast genome sequences provide more integrated and adequate information for better understanding the phylogenetic pattern and improving efficient discrimination during species identification.
巴黎因其药用价值而在中国闻名，并已被列入《中国药典》。这些物种的鉴定不准确可能会影响它们的有效探索、保护和驯化。由于形态特征的可塑性，正确鉴定巴黎物种仍然是一个问题。在这方面，我们报告了 P. thibetica 和 P. rugosa 的完整叶绿体基因组，以开发高度可变的分子标记。通过比较三个叶绿体基因组，我们寻找最具变异性的区域，为巴黎开发最佳的 cpDNA 条形码。巴黎叶绿体基因组的大小范围为 162708 到 163200bp。在所有三个叶绿体基因组中都观察到了 134 个基因，包括 81 个蛋白质编码基因、45 个 tRNA 基因和 8 个 rRNA 基因。检测到了 8 个快速进化区域，以及简单重复序列（SSR）和重复序列的差异。编码基因 ycf1、ycf1a 和 ycf1b 的两个区域进化最快，被提议作为巴黎的核心条形码。完整的叶绿体基因组序列为更好地理解系统发育模式和提高物种鉴定中的有效鉴别提供了更综合和充分的信息。
