Tuomanen E, Weiss A
J Infect Dis. 1985 Jul;152(1):118-25. doi: 10.1093/infdis/152.1.118.
Bordetella pertussis, the causative agent of whooping cough, adheres specifically to human ciliated respiratory-epithelial cells. In order to identify the adhesins of Bordetella, we tested mutant strains, each deficient in one of several bacterial virulence factors, for the ability to adhere to single, human ciliated cells in vitro. Loss of adherence was associated with lack of secretion of either of two surface antigens: filamentous hemagglutinin or pertussis toxin. These strains regained adherence if both components were present in the assay system (i.e., the missing protein was supplied exogenously). In this instance, both bacterial components were found bound to the surface of all the Bordetella strains tested as well as to cilia. We hypothesize that filamentous hemagglutinin and pertussis toxin, two naturally secreted surface antigens, act in concert as adhesins of Bordetella for human cilia.
百日咳博德特氏菌是百日咳的病原体，它能特异性地黏附于人类有纤毛的呼吸道上皮细胞。为了鉴定博德特氏菌的黏附素，我们测试了几种突变菌株，每种突变菌株都缺乏几种细菌毒力因子中的一种，以检测它们在体外黏附单个人类有纤毛细胞的能力。黏附能力的丧失与两种表面抗原（丝状血凝素或百日咳毒素）中任何一种的分泌缺失有关。如果检测系统中同时存在这两种成分（即缺失的蛋白质通过外源供应），这些菌株就能恢复黏附能力。在这种情况下，发现这两种细菌成分都结合在所有测试的博德特氏菌菌株表面以及纤毛上。我们推测，丝状血凝素和百日咳毒素这两种天然分泌的表面抗原协同作用，作为博德特氏菌对人类纤毛的黏附素。
