Liu Si-Jing, Jiang Ming-Juan, Pu Qi-Kang, Ren Chen-Yan, Su Lin, Zhang Xiang, Wang Chuan
Department of Public Health Laboratory Sciences, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 Mar;48(2):244-247.
To predict and analyze the antigenic epitopes in protein caseinolytic protease P2 (clpP2), and explore its possibility to be applied as a new tuberculosis (TB) vaccine and drug development target.
Secondary structure of clpP2 based on nucleic sequence was predicted by DNA Star software. The homologous sequence conformation were analyzed by Swiss-Model online software. T cells antigenic epitopes were predicted through VaxiPred, and B cell epitopes were predicted by combining use of several different prediction programs, such as ABCpred, COBEPro and BepiPredPred. The immune characteristics of clpP2 were analyzed by DNA Star, SignalP, TMHMM online software and were searched through NCBI database.
protein was diverse in structure, composing with a great deal of CTL and Th cell epitopes. clpP2 was also predicted to comprise rich potential liner and discontinuous B-cell epitopes. These epitopes were accessible on the protein surface, located in flexible and hydrophilic regions.
clpP2 is prompted to induce immune responses and developes a novel target in surveillance, treatment and vaccine.
预测并分析酪蛋白水解蛋白酶P2（clpP2）中的抗原表位，探讨其作为新型结核病（TB）疫苗及药物研发靶点的可能性。
采用DNA Star软件根据核酸序列预测clpP2的二级结构。通过在线软件Swiss - Model分析同源序列构象。利用VaxiPred预测T细胞抗原表位，联合使用ABCpred、COBEPro和BepiPredPred等多种不同预测程序预测B细胞表位。借助DNA Star、SignalP、TMHMM在线软件分析clpP2的免疫特性，并通过NCBI数据库进行检索。
该蛋白结构多样，包含大量细胞毒性T淋巴细胞（CTL）和辅助性T细胞（Th）表位。预测clpP2还包含丰富的潜在线性和不连续B细胞表位。这些表位位于蛋白质表面，处于灵活且亲水的区域。
提示clpP2可诱导免疫反应，并成为监测、治疗及疫苗研发的新靶点。
