[结核分枝杆菌α-晶体蛋白（Rv2031c）的T细胞和B细胞表位的在线分析]

[On-line analysis of T and B cell epitopes of α-crystallin (Rv2031c) derived from Mycobacterium tuberculosis].

作者信息

Liu Na, Meng Xiangying, Li Heng, Qiao Injuan

机构信息

Weifang Medical University, Weifang 261053, China.

Weifang Medical University, Weifang 261053, China. *Corresponding author, E-mail:

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 May;34(5):408-413.

PMID:30043731

Abstract

Objective To predict the epitopes of Mycobacterium tuberculosis dormancy-related protein α-crystallin (Rv2031c). Methods The homology between Rv2031c and human proteins was analyzed online by BLAST alignment. B- and T-cell epitopes of Rv2031c were predicted by Protean of DNAStar software. RANKPEP and SYPEPITHI were used to predict the epitopes of T helper (Th) cells; while SYFPEPI, BIMAS, and NetCTL were used to predict the epitopes of cytotoxic T lymphocytes (CTLs). Results Rv2031c had low homology with human proteins. Eight potential epitopes of B-cells, 7 epitopes of Th cells and 3 epitopes of CTLs were predicted in Rv2031c. Conclusion Rv2031c, which has many potential epitopes of Th cells, CTLs and B-cells, is expected to be a promising candidate for the development of tuberculosis vaccines.

摘要

目的预测结核分枝杆菌休眠相关蛋白α-晶体蛋白（Rv2031c）的表位。方法通过BLAST比对在线分析Rv2031c与人蛋白质之间的同源性。利用DNAStar软件的Protean预测Rv2031c的B细胞和T细胞表位。使用RANKPEP和SYPEPITHI预测辅助性T（Th）细胞表位；而使用SYFPEPI、BIMAS和NetCTL预测细胞毒性T淋巴细胞（CTL）表位。结果 Rv2031c与人蛋白质的同源性较低。在Rv2031c中预测到8个潜在的B细胞表位、7个Th细胞表位和3个CTL表位。结论 Rv2031c具有许多Th细胞、CTL和B细胞的潜在表位，有望成为开发结核病疫苗的有前景的候选物。