Chaudhary Dhiraj Kumar, Dahal Ram Hari, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1820-1826. doi: 10.1099/ijsem.0.001869. Epub 2017 Jun 14.
A yellow-coloured, Gram-staining-negative, motile and rod shaped bacterium, designated strain R366T, was isolated from forest soil of Kyonggi University, South Korea. It was able to grow at 15-42 °C, pH 6.0-10.0 and with 0-4 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain R366T belongs to the genus Sphingopyxis and is closely related to Sphingopyxis italica SC13E-S71T (98.72 % sequence similarity), Sphingopyxis chilensis S37T (98.51 %), Sphingopyxis fribergensis Kp5.2T (98.29 %), Sphingopyxis alaskensis RB2256T (98.15 %), Sphingopyxis ginsengisoli Gsoli 250T (98.15 %) and Sphingopyxis taejonensis JSS54T (98.01 %). The predominant respiratory quinone was ubiquinone-10, and the major polyamine was spermidine. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine and sphingoglycolipid. The predominant fatty acids of strain R366T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C17 : 1ω6c, C16 : 0, C18 : 1ω7c 11-methyl and C14 : 0 2-OH. The genomic DNA G+C content of this novel strain was 65.1 mol%. The DNA-DNA relatedness between strain R366T and Sphingopyxisitalica DSM 25229T, Sphingopyxischilensis KCCM 41918T, Sphingopyxisalaskensis KCCM 41983T, Sphingopyxisginsengisoli KACC 13918T and Sphingopyxistaejonensis KACC 12341T was 51.7, 45.3, 39.0, 41.3 and 44.7 %, respectively. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain R366T represents a novel species of the genus Sphingopyxis, for which the name Sphingopyxis solisilvae sp. nov. is proposed. The type strain is R366T (=KEMB 9005-451T=KACC 19003T=JCM 31675T).
从韩国庆熙大学的森林土壤中分离出一株黄色、革兰氏染色阴性、具运动性的杆状细菌，命名为菌株R366T。它能够在15 - 42 °C、pH 6.0 - 10.0以及0 - 4 %（w/v）NaCl浓度下生长。基于16S rRNA基因序列分析，菌株R366T属于鞘氨醇单胞菌属，与意大利鞘氨醇单胞菌SC13E - S71T（序列相似性为98.72 %）、智利鞘氨醇单胞菌S37T（98.51 %）、弗里贝格鞘氨醇单胞菌Kp5.2T（98.29 %）、阿拉斯加鞘氨醇单胞菌RB2256T（98.15 %）、人参鞘氨醇单胞菌Gsoli 250T（98.15 %）和大田鞘氨醇单胞菌JSS54T（98.01 %）密切相关。主要呼吸醌为泛醌 - 10，主要多胺为亚精胺。极性脂质谱显示存在磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油、磷脂酰胆碱、磷脂酰单甲基乙醇胺和鞘糖脂。菌株R366T的主要脂肪酸为总和特征8（C18 : 1ω7c和/或C18 : 1ω6c）、总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）、C17 : 1ω6c、C16 : 0、C18 : 1ω7c 11 - 甲基和C14 : 0 2 - 羟基。该新菌株的基因组DNA G + C含量为65.1 mol%。菌株R366T与意大利鞘氨醇单胞菌DSM 25229T、智利鞘氨醇单胞菌KCCM 41918T、阿拉斯加鞘氨醇单胞菌KCCM 41983T、人参鞘氨醇单胞菌KACC 13918T和大田鞘氨醇单胞菌KACC 12341T的DNA - DNA相关性分别为51.7、45.3、39.0、41.3和44.7 %。形态学、生理学、化学分类学和系统发育分析清楚地将该菌株与其最接近的系统发育邻居区分开来。因此，菌株R366T代表鞘氨醇单胞菌属的一个新物种，为此提出名为Sphingopyxis solisilvae sp. nov.的名称。模式菌株为R366T（=KEMB 9005 - 451T=KACC 19003T=JCM 31675T）。
