Washington University School of Medicine, Mallinckrodt Institute of Radiology, Optical Radiology Laboratory, St. Louis, Missouri, United StatesbWashington University in St. Louis, Biomedical Engineering, St. Louis, Missouri, United States.
Washington University School of Medicine, Mallinckrodt Institute of Radiology, Optical Radiology Laboratory, St. Louis, Missouri, United States.
J Biomed Opt. 2017 Jun 1;22(6):66007. doi: 10.1117/1.JBO.22.6.066007.
Similarity of skin cancer with many benign skin pathologies requires reliable methods to detect and differentiate the different types of these lesions. Previous studies have explored the use of disparate optical techniques to identify and estimate the invasive nature of melanoma and basal cell carcinoma with varying outcomes. Here, we used a concerted approach that provides complementary information for rapid screening and characterization of tumors, focusing on squamous cell carcinoma (SCC) of the skin. Assessment of in vivo autofluorescence lifetime (FLT) imaging of endogenous fluorophores that are excitable at longer wavelengths (480 nm) than conventional NADH and FAD revealed a decrease in the short FLT component for SCC compared to normal skin, with mean values of 0.57 ± 0.026 ?? ns and 0.61 ± 0.021 ?? ns , respectively ( p = 0.004 ). Subsequent systemic administration of a near-infrared fluorescent molecular probe in SCC bearing mice, followed by the implementation of image processing methods on data acquired from two-dimensional and three-dimensional fluorescence molecular imaging, allowed us to estimate the tumor volume and depth, as well as quantify the fluorescent probe in the tumor. The result suggests the involvement of lipofuscin-like lipopigments and riboflavin in SCC metabolism and serves as a model for staging SCC.
皮肤癌与许多良性皮肤病理具有相似性，因此需要可靠的方法来检测和区分这些病变的不同类型。先前的研究已经探索了使用不同的光学技术来识别和估计黑色素瘤和基底细胞癌的侵袭性，但其结果存在差异。在这里，我们使用了一种协同方法，该方法为肿瘤的快速筛查和特征提供了互补信息，重点关注皮肤鳞状细胞癌 (SCC)。评估体内可激发长波长（480nm）的内源性荧光团的自发荧光寿命 (FLT) 成像，与正常皮肤相比，SCC 的短 FLT 分量降低，平均值分别为 0.57 ± 0.026 ns 和 0.61 ± 0.021 ns ( p = 0.004 )。随后在患有 SCC 的小鼠中系统给予近红外荧光分子探针，并对从二维和三维荧光分子成像中获取的数据实施图像处理方法，使我们能够估计肿瘤的体积和深度，并定量测定肿瘤中的荧光探针。结果表明，类脂褐素样脂色素和核黄素参与了 SCC 的新陈代谢，可作为 SCC 分期的模型。
