Düsman E, Almeida I V, Pinto E P, Lucchetta L, Vicentini V E P
Departamento Acadêmico de Química e Biologia, Universidade Tecnológica Federal do Paraná, Francisco Beltrão, PR, Brasil
Departamento de Biotecnologia, Genética e Biologia Celular, , , Brasil.
Genet Mol Res. 2017 May 31;16(2):gmr-16-02-gmr.16029670. doi: 10.4238/gmr16029670.
Integral grape juice is extracted from the grape through processes that allow the retention of their natural composition. However, due to the severity of some processes, fruit juices can undergo changes in their quality. The present study evaluated the cytotoxic and mutagenic effects of integral grape juice by a cytokinesis-blocked micronucleus assay in Rattus norvegicus hepatoma cells (HTC) in vitro. Vitis labrusca L. (variety Concord) were produced organically and by a conventional system, and their juice was extracted by a hot extraction process. The organic grapes were subjected to ultraviolet-type C radiation (UV-C). Experiments were performed after production and after 6 months in storage. Physicochemical analyses revealed that UV-C irradiation of organic grapes, the juice production process, and storage resulted in nutraceutical alterations. However, none of the juice concentrations were cytotoxic to HTC cells by the cytokinesis-blocked proliferation index results or were mutagenic, because the formation of micronucleated cells was not induced. In general, juice induced cell proliferation, possibly due to the presence of vitamins and sugar content (total soluble solid). The data increased the understanding of food technology and confirmed the quality and safety consumption of these juices.
完整葡萄汁是通过能保留其天然成分的工艺从葡萄中提取的。然而，由于某些工艺的严苛性，果汁的质量可能会发生变化。本研究通过体外胞质分裂阻滞微核试验，评估了完整葡萄汁对褐家鼠肝癌细胞（HTC）的细胞毒性和诱变作用。美洲葡萄（康科德品种）采用有机方式和传统系统种植，其果汁通过热提取工艺提取。对有机葡萄进行了C型紫外线（UV-C）辐射。在生产后和储存6个月后进行了实验。理化分析表明，对有机葡萄进行UV-C辐射、果汁生产工艺以及储存导致了营养成分的改变。然而，根据胞质分裂阻滞增殖指数结果，没有一种果汁浓度对HTC细胞具有细胞毒性，也没有诱变作用，因为未诱导微核细胞的形成。总体而言，果汁可诱导细胞增殖，这可能是由于维生素和糖分含量（总可溶性固形物）的存在。这些数据增进了对食品技术的理解，并证实了这些果汁的质量和食用安全性。
