3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
INL-International Iberian Nanotechnology Laboratory, Braga, Portugal.
Nanomedicine. 2018 Oct;14(7):2375-2385. doi: 10.1016/j.nano.2017.06.002. Epub 2017 Jun 11.
Photocrosslinkable magnetic hydrogels are attracting great interest for tissue engineering strategies due to their versatility and multifunctionality, including their remote controllability ex vivo, thus enabling engineering complex tissue interfaces. This study reports the development of a photocrosslinkable magnetic responsive hydrogel made of methacrylated chondroitin sulfate (MA-CS) enriched with platelet lysate (PL) with tunable features, envisioning their application in tendon-to-bone interface. MA-CS coated iron-based magnetic nanoparticles were incorporated to provide magnetic responsiveness to the hydrogel. Osteogenically differentiated adipose-derived stem cells and/or tendon-derived cells were encapsulated within the hydrogel, proliferating and expressing bone- and tendon-related markers. External magnetic field (EMF) application modulated the swelling, degradation and release of PL-derived growth factors, and impacted both cell morphology and the expression and synthesis of tendon- and bone-like matrix with a more evident effect in co-cultures. Overall, the developed magnetic responsive hydrogel represents a potential cell carrier system for interfacial tissue engineering with EMF-controlled properties.
光交联磁性水凝胶因其多功能性和通用性，包括其体外的远程可控性，从而能够工程复杂的组织界面，在组织工程策略中引起了极大的兴趣。本研究报告了一种由富含血小板裂解液 (PL) 的甲基丙烯酰化硫酸软骨素 (MA-CS) 制成的光交联磁响应水凝胶的开发，该水凝胶具有可调特性，设想将其应用于肌腱-骨界面。将涂覆有铁基磁性纳米粒子的 MA-CS 掺入水凝胶中，赋予水凝胶磁响应性。成骨分化的脂肪来源干细胞和/或肌腱来源细胞被包裹在水凝胶内，增殖并表达与骨和肌腱相关的标志物。外加磁场 (EMF) 的应用调节了 PL 衍生生长因子的溶胀、降解和释放，并影响了细胞形态以及肌腱和骨样基质的表达和合成，在共培养中效果更为明显。总的来说，开发的磁响应水凝胶代表了一种具有 EMF 控制特性的界面组织工程的潜在细胞载体系统。
