Nam Sun-Young, Kim Kyu-Yeob, Kim Mi Hye, Jang Jae-Bum, Rah So-Young, Chae Hee Jeong, Lee Jin-Man, Kim Hyung-Min, Jeong Hyun-Ja
a Department of Pharmacology, College of Korean Medicine , Kyung Hee University , Seoul , Republic of Korea.
b Department of Food and Nutrition , Hoseo University , Asan , Chungnam , Republic of Korea.
Pharm Biol. 2017 Dec;55(1):1856-1862. doi: 10.1080/13880209.2017.1339282.
To study the anti-inflammatory properties of OJ.
Ojayeonjonghwan (OJ) is a traditional Korean prescription, which has been widely used for the treatment of prostatitis. However, no scientific study has been performed of the anti-inflammatory effects of OJ.
Peritoneal macrophages were isolated 3-4 days after injecting a C57BL/6J mouse with thioglycollate. They were then treated with OJ water extract (0.01, 0.1, and 1 mg/mL) for 1 h and stimulated with lipopolysaccharide (LPS) for different times. Nitric oxide (NO), inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and proinflammatory cytokine levels were determined by NO assay, Western blotting, RT-PCR and ELISA.
NO generation and iNOS induction were increased in the LPS-activated mouse peritoneal macrophages. However, NO generation and iNOS induction by LPS were suppressed by treatment with OJ for the first time. The IC value of OJ with respect to NO production was 0.09 mg/mL. OJ did not influence LPS-stimulated COX-2 induction, but did significantly decrease LPS-stimulated secretions and mRNA expressions of tumour necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β. Inhibition rates of TNF-α, IL-6, and IL-1β at an OJ concentration of 1 mg/mL were 77%, 88%, and 50%, respectively. OJ also suppressed the LPS-induced nuclear translocation of NF-κB. High-performance liquid chromatography showed schizandrin and gomisin A are major components of OJ.
OJ reduces inflammatory response, and this probably explains its positive impact on the prostatitis associated inflammation.
研究OJ的抗炎特性。
Ojayeonjonghwan（OJ）是一种传统的韩国方剂，已被广泛用于治疗前列腺炎。然而，尚未对OJ的抗炎作用进行科学研究。
在给C57BL/6J小鼠注射巯基乙酸盐3 - 4天后分离腹腔巨噬细胞。然后用OJ水提取物（0.01、0.1和1mg/mL）处理1小时，并用脂多糖（LPS）刺激不同时间。通过NO测定、蛋白质印迹、逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）测定一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）和环氧化酶（COX）-2以及促炎细胞因子水平。
在LPS激活的小鼠腹腔巨噬细胞中，NO生成和iNOS诱导增加。然而，首次发现用OJ处理可抑制LPS诱导的NO生成和iNOS诱导。OJ对NO产生的半数抑制浓度（IC值）为0.09mg/mL。OJ不影响LPS刺激的COX-2诱导，但确实显著降低LPS刺激的肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6和IL-1β的分泌及mRNA表达。在OJ浓度为1mg/mL时，TNF-α、IL-6和IL-1β的抑制率分别为77%、88%和50%。OJ还抑制LPS诱导的核因子κB（NF-κB）核转位。高效液相色谱显示五味子醇甲和戈米辛A是OJ的主要成分。
OJ可减轻炎症反应，这可能解释了其对前列腺炎相关炎症的积极影响。
