Santos Bruna Parapinski, Souza Fernando Nogueira, Blagitz Maiara Garcia, Batista Camila Freitas, Bertagnon Heloísa Godoi, Diniz Soraia Araújo, Silva Marcos Xavier, Haddad João Paulo Amaral, Della Libera Alice Maria Melville Paiva
Veterinary Clinical Immunology Research Group, Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, Brazil.
Veterinary Clinical Immunology Research Group, Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, Brazil.
Vet Immunol Immunopathol. 2017 Jun;188:21-26. doi: 10.1016/j.vetimm.2017.04.009. Epub 2017 Apr 29.
The exact influence of caprine arthritis encephalitis virus (CAEV) infection on blood and milk polymorphonuclear leukocytes (PMNLs) and monocyte/macrophages of goats remains unclear. Thus, the present study sought to explore the blood and milk PMNL and monocyte/macrophage functions in naturally CAEV-infected goats. The present study used 18 healthy Saanen goats that were segregated according to sera test outcomes into serologically CAEV negative (n=8; 14 halves) and positive (n=10; 14 halves) groups. All milk samples from mammary halves with milk bacteriologically positive outcomes, somatic cell count ≥2×10cellsmL, and abnormal secretions in the strip cup test were excluded. We evaluated the percentage of blood and milk PMNLs and monocyte/macrophages, the viability of PMNLs and monocyte/macrophages, the levels of intracellular reactive oxygen species (ROS) and the nonopsonized phagocytosis of Staphylococcus aureus and Escherichia coli by flow cytometry. In the present study, a higher percentage of milk macrophages (CD14) and milk polymorphonuclear leukocytes undergoing late apoptosis or necrosis (Annexin-V/Propidium iodide) was observed in CAEV-infected goats; we did not find any further alterations in blood and milk PMNL and monocyte/macrophage functions. Thus, regarding our results, the goats naturally infected with CAEV did not reveal pronounced dysfunctions in blood and milk polymorphonuclear leukocytes and monocytes/macrophages.
山羊关节炎脑炎病毒（CAEV）感染对山羊血液和乳汁中的多形核白细胞（PMNLs）以及单核细胞/巨噬细胞的确切影响尚不清楚。因此，本研究旨在探索自然感染CAEV的山羊血液和乳汁中的PMNL以及单核细胞/巨噬细胞的功能。本研究使用了18只健康的萨能山羊，根据血清检测结果将其分为血清学CAEV阴性组（n = 8；14个乳腺半区）和阳性组（n = 10；14个乳腺半区）。所有来自乳腺半区且乳汁细菌学检测呈阳性、体细胞计数≥2×10⁵个/mL以及乳汁杯试验中出现异常分泌物的乳汁样本均被排除。我们通过流式细胞术评估了血液和乳汁中PMNL以及单核细胞/巨噬细胞的百分比、PMNL以及单核细胞/巨噬细胞的活力、细胞内活性氧（ROS）水平以及金黄色葡萄球菌和大肠杆菌的非调理吞噬作用。在本研究中，在感染CAEV的山羊中观察到乳汁巨噬细胞（CD14）以及经历晚期凋亡或坏死的乳汁多形核白细胞（膜联蛋白V/碘化丙啶）的百分比更高；我们未发现血液和乳汁中PMNL以及单核细胞/巨噬细胞功能有任何进一步的改变。因此，基于我们的研究结果，自然感染CAEV的山羊在血液和乳汁多形核白细胞以及单核细胞/巨噬细胞方面未表现出明显的功能障碍。
