Rimstad E, East N E, Torten M, Higgins J, DeRock E, Pedersen N C
Department of Medicine, School of Veterinary Medicine, University of California, Davis 95616.
Am J Vet Res. 1993 Nov;54(11):1858-62.
One hundred eight milking goats from a dairy that had been using a modified caprine arthritis-encephalitis virus (CAEV) eradication program were tested for CAEV antibodies by serologic methods and for proviral CAEV DNA by use of polymerase chain reaction (PCR) technology. All goats were free of clinical symptoms of CAEV infection. Twenty-seven of the 108 goats were considered seropositive, on the basis of ELISA results. Proviral CAEV DNA was detected, using PCR techniques, in mononuclear leukocytes in blood samples obtained from 25 of the these 27 seropositive goats. Twenty of the 81 seronegative goats also had positive PCR test results. Ten of these goats seroconverted by 8 months later, and virus was readily isolated from mononuclear leukocytes in venous blood samples after the goats had seroconverted. Virus was also isolated from mononuclear leukocytes in blood samples collected from 4 of 11 goats that were seronegative, but had positive PCR test results. These results indicated that seroconversion can be delayed for many months following natural infection with CAEV. Delayed seroconversion appears to be a feature of CAEV infection, which may have direct implications for CAEV eradication programs and epidemiologic studies that rely on serologic methods to detect infected goats.
对一家采用改良山羊关节炎-脑炎病毒（CAEV）根除计划的奶牛场的108只产奶山羊，通过血清学方法检测CAEV抗体，并使用聚合酶链反应（PCR）技术检测前病毒CAEV DNA。所有山羊均无CAEV感染的临床症状。根据ELISA结果，108只山羊中有27只被认为血清学呈阳性。使用PCR技术，在从这27只血清学阳性山羊中的25只采集的血液样本的单核白细胞中检测到前病毒CAEV DNA。81只血清学阴性的山羊中有20只PCR检测结果也为阳性。其中10只山羊在8个月后血清转化，在山羊血清转化后，很容易从静脉血样本的单核白细胞中分离出病毒。在11只血清学阴性但PCR检测结果为阳性的山羊中，有4只的血液样本的单核白细胞中也分离出了病毒。这些结果表明，自然感染CAEV后血清转化可能会延迟数月。延迟血清转化似乎是CAEV感染的一个特征，这可能对CAEV根除计划以及依靠血清学方法检测感染山羊的流行病学研究有直接影响。
