[维生素和各种氨基酸对浅灰链霉菌培养物合成葡萄糖异构酶的影响]

[Effect of vitamins and various amino acids on glucose isomerase biosynthesis by Streptomyces albogriseolus culture].

作者信息

Gavristov A V, Afanas'eva V P, Zhitskaia E A, Golovina N S, Nakhapetian L A

出版信息

Mikrobiologiia. 1985 Jan-Feb;54(1):55-61.

Abstract

The effect of vitamins (B1, B2, B3, B6, B12, H, PP and folic acid) and amino acids (glutamic and aspartic acids) on glucose isomerase biosynthesis was studied in Streptomyces albogriseolus. These compounds were added either alone or in combinations to different growth media (synthetic and complex). The results were processed using mathematical methods, and the following mixture of vitamins and amino acids was proposed to be added to the complex fermentation medium: B2, 10 micrograms/L; B6, 10 micrograms/L; H, 1 microgram/L; aspartic acid, 0.01 microM. The production of glucose isomerase rose more than 1.5 times after such additions.

摘要

在浅灰链霉菌中研究了维生素（维生素B1、B2、B3、B6、B12、生物素、维生素PP和叶酸）和氨基酸（谷氨酸和天冬氨酸）对葡萄糖异构酶生物合成的影响。这些化合物单独或组合添加到不同的生长培养基（合成培养基和复合培养基）中。使用数学方法对结果进行处理，建议向复合发酵培养基中添加以下维生素和氨基酸混合物：维生素B2，10微克/升；维生素B6，10微克/升；生物素，1微克/升；天冬氨酸，0.01微摩尔。添加这些物质后，葡萄糖异构酶的产量提高了1.5倍以上。