Domańska-Janik K, Lazarewicz J, Noremberg K, Strosznajder J, Zalewska T
Neurochem Res. 1985 May;10(5):649-65. doi: 10.1007/BF00964404.
The effects of cerebral ischemia, induced for 10 min by bilateral common carotid ligation in the Mongolian gerbil, on the brain and synaptosomal content of phospholipids and free fatty acids were measured. Moreover, the incorporation of arachidonic acid and oleoyl-CoA into phospholipids, as well as the respiration and the accumulation of 45Ca, norepinephrine, dopamine, choline, glutamate, and gamma-aminobutyrate in the ischemic brain synaptosomal fraction were studied. Analyses of lipids showed a drop in phospholipids content with concomitant increase of lysocompounds and free fatty acids in ischemic cerebral cortex. Disturbances in lipid metabolism including rapid phospholipids hydrolysis and changes in the incorporation of arachidonic acid into inositol and choline phosphoglycerides were also shown in the synaptosomal fraction of ischemic brain. The uptake of neurotransmitter substances, expressed as a percent of control value, was reduced 21% for norepinephrine, 40% for dopamine, 20% for choline, 24% for glutamate and 13% for gamma-aminobutyrate in ischemic synaptosomes. There was no significant effect of ischemia on synaptosomal respiration and 45Ca uptake in both control and high potassium media. The inhibition of neurotransmitter uptake in ischemic brain synaptosomes may be caused by the disturbance of fatty acid metabolism.
通过双侧颈总动脉结扎蒙古沙鼠10分钟诱导脑缺血，测量其对脑及突触体中磷脂和游离脂肪酸含量的影响。此外，还研究了花生四烯酸和油酰辅酶A掺入磷脂的情况，以及缺血脑突触体组分中45Ca、去甲肾上腺素、多巴胺、胆碱、谷氨酸和γ-氨基丁酸的呼吸作用和积累。脂质分析显示，缺血性大脑皮层中磷脂含量下降，同时溶血化合物和游离脂肪酸增加。缺血脑突触体组分中还显示出脂质代谢紊乱，包括磷脂快速水解以及花生四烯酸掺入肌醇和胆碱磷酸甘油酯的变化。在缺血性突触体中，神经递质物质的摄取量（以对照值的百分比表示），去甲肾上腺素降低了21%，多巴胺降低了40%，胆碱降低了20%，谷氨酸降低了24%，γ-氨基丁酸降低了13%。在对照和高钾培养基中，缺血对突触体呼吸作用和45Ca摄取均无显著影响。缺血性脑突触体中神经递质摄取的抑制可能是由脂肪酸代谢紊乱引起的。
