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基于形态测量和扫描电子显微镜技术对犬体内寄生虫的鉴定

Morphometric and scanning electron microscopy based identification of parasites in dog.

作者信息

Uppal Harsimarandeep Singh, Bal Mandeep Singh, Singla L D, Kaur Paramjit, Sandhu B S

机构信息

Department of Veterinary Parasitology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.

Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.

出版信息

J Parasit Dis. 2017 Jun;41(2):517-522. doi: 10.1007/s12639-016-0841-y. Epub 2016 Oct 7.

Abstract

, a blood feeding nematode parasite (Family: Ancylostomatidae, Superfamily: Ancylostomatoidea) can cause anaemia, dark reddish-brown to black haemorrhagic diarrhoea, dehydration, wasting and deaths due to heavy blood loss. Adult hook worm parasites recovered from the intestine of a stray dog at the time of necropsy were identified as based on morphological characters and morphometric observations involving scanning electron microscopy (SEM). Different developmental stages of hookworm eggs viz. 8 cell stage, morula, gastrula and vermiform were observed during the culture process of faecal sample. High quality SEM photographs showed teeth of dimensions 52.5, 42.3 and 23.5 μm on one side and 55.4, 43.8 and 21.0 μm on the other side along with the presence of characteristic transverse cuticular striations on body surface of parasites.

摘要

一种吸血线虫寄生虫(家族:钩口科,总科:钩口总科)可导致贫血、深红褐色至黑色出血性腹泻、脱水、消瘦以及因大量失血而死亡。在尸检时从一只流浪狗的肠道中回收的成年钩虫寄生虫,根据形态特征和包括扫描电子显微镜(SEM)在内的形态测量观察结果,被鉴定为[具体种类未给出]。在粪便样本的培养过程中,观察到了钩虫卵的不同发育阶段,即8细胞期、桑椹胚、原肠胚和蠕虫状。高质量的扫描电子显微镜照片显示,[具体种类未给出]寄生虫体表一侧的齿尺寸为52.5、42.3和23.5微米,另一侧为55.4、43.8和21.0微米,同时还存在特征性的横向表皮条纹。

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