Mishra Sapna, Kumar Peeyush, Malik Anushree
Applied Microbiology laboratory, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi, 110 016 India.
J Parasit Dis. 2017 Jun;41(2):543-550. doi: 10.1007/s12639-016-0848-4. Epub 2016 Sep 29.
Microscopic investigation was done to determine the effect of entomopathogenic fungi, and essential oil of on different life stages of . Scanning electron microscopy investigation of fungal infected larvae showed sluggish movement, rigor, and failure of body to balance in water. Treated larvae also revealed varied level of cuticle shrinkage and extreme dehydration. Surface of infected pupae showed varied stage of mycelial growth, while the cadaver of adult fly was observed to have extensive fungal growth covering their entire body surface. The application of oils on larvae resulted in skin shrinkage, spinous cells proliferation and bleb formation, while the treated pupae showed high incidence of incomplete emergence and malformation in emerged adult flies. The current study establishes effect of essential oil and infection on different life stages of .
进行了显微镜检查以确定昆虫病原真菌和精油对[昆虫名称]不同生命阶段的影响。对真菌感染幼虫的扫描电子显微镜检查显示其运动迟缓、僵硬,且在水中身体无法保持平衡。经处理的幼虫还表现出不同程度的表皮收缩和极度脱水。被感染蛹的表面显示出不同阶段的菌丝生长,而成蝇尸体则观察到有广泛的真菌生长覆盖其整个体表。将[精油名称]油施用于[昆虫名称]幼虫导致皮肤收缩、棘状细胞增殖和气泡形成,而经处理的蛹在羽化出的成蝇中显示出不完全羽化和畸形的高发生率。当前研究确定了[精油名称]精油和[真菌名称]感染对[昆虫名称]不同生命阶段的影响。