与抗Y29/55单克隆抗体反应的B淋巴细胞的组织分布及超微结构

Tissue distribution and ultrastructure of B lymphocytes reacting with the monoclonal antibody anti-Y29/55.

作者信息

Gudat F G, Forster H K, Suter F, Albrecht R, Krey G, Dürmüller U, Girard M F, Obrecht J P

出版信息

Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;48(4):341-50. doi: 10.1007/BF02890140.

Abstract

The reactivity of normal tonsilar cells with the monoclonal antibody anti-Y29/55 is characterized at the tissue and ultrastructural cytological level. Using an indirect immuno-alkaline phosphatase method on frozen sections the antibody labels mantle zone and germinal center lymphocytes. This staining reaction is more generalized in B-lymphocyte areas than that obtained with antibodies to IgM and IgD. By indirect immunoperoxidase staining, as well as by an indirect rosetting procedure in cell suspensions, the reactive cell population were either small resting lymphocytes or activated lymphocytes corresponding to centrocytes, centroblasts, immunoblasts and plasmoblasts; some plasma cells were also labeled. These results characterize the monoclonal antibody anti-Y29/55 as a pan-B-marker antibody, useful for labeling resting and activated peripheral B-lymphocytes in frozen tissue sections and cell suspensions.

摘要

在组织和超微结构细胞学水平上对正常扁桃体细胞与抗Y29/55单克隆抗体的反应性进行了表征。在冰冻切片上使用间接免疫碱性磷酸酶方法，该抗体标记套区和生发中心淋巴细胞。这种染色反应在B淋巴细胞区域比用抗IgM和IgD抗体获得的反应更普遍。通过间接免疫过氧化物酶染色以及细胞悬液中的间接花环形成程序，反应性细胞群体要么是小的静止淋巴细胞，要么是对应于中心细胞、中心母细胞、免疫母细胞和成浆细胞的活化淋巴细胞；一些浆细胞也被标记。这些结果表明抗Y29/55单克隆抗体是一种泛B标记抗体，可用于标记冰冻组织切片和细胞悬液中的静止和活化外周B淋巴细胞。