Qu Bo, Yu Fei, Sheng Guan-Nan, Chen Guan-Nan, Lü Qi, Gu Yan-Jun, Guo Long
Department of Surgical Oncology, Affiliated Hospital of Logistics University of PAPF, Training Base of Jinzhou Medical University, Tianjin 300162, China.
Department of Hepatobiliary Surgery, the First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 May;48(3):373-377.
To investigate the expression of migration-inducing gene 7 (MIG7) in different HCC lines and its relationship with vasculogenic mimicry (VM) formation and metastatic potentiality.
Employing immunostaining to detect MIG7 protein expression and VM formation in 40 matched pairs of primary and metastatic HCC specimens from 40 patients, and investigating the correlation of VM formation with MIG7 protein expression. Detecting VM formation in HCC lines with different metastatic ability (MHCC-97H, MHCC-97L, Huh-7) and human normal hepatocyte line (L-02) through three-dimensional culture, and detecting mRNA expression with RT-PCR, investigating the correlation of MIG7 protein expression with VM formation and HCC metastatic potentiality with Western blot assay; screening the HCC cell line with high MIG7 expression.
In 40 matched pairs of HCC tissue, there was a significant positive correlation between MIG7 protein expression and VM formation ( =0.595, <0.01). The capability of VM formation of MHCC-97H with high metastatic potentiality was stronger than that of MHCC-97L with low metastatic potentiality and Huh-7 with non-metastatic potentiality, and there was no VM formation in L-02. The result of RT-PCR and Western blot assay indicated the same.
MIG7 expression in HCC tissue is high and correlated positively with VM formation. MIG7 expression in different HCC cell lines is coincident with theirs VM formation, invasion and metastasis. MIG7 is a potential target for inhibiting the invasion and metastasis of HCC.
研究迁移诱导基因7（MIG7）在不同肝癌细胞系中的表达及其与血管生成拟态（VM）形成和转移潜能的关系。
采用免疫染色检测40例患者40对原发性和转移性肝癌标本中MIG7蛋白表达及VM形成情况，并研究VM形成与MIG7蛋白表达的相关性。通过三维培养检测不同转移能力的肝癌细胞系（MHCC-97H、MHCC-97L、Huh-7）和人正常肝细胞系（L-02）中的VM形成情况，采用RT-PCR检测mRNA表达，通过蛋白质印迹法研究MIG7蛋白表达与VM形成及肝癌转移潜能的相关性；筛选高表达MIG7的肝癌细胞系。
在40对肝癌组织中，MIG7蛋白表达与VM形成之间存在显著正相关（=0.595，<0.01）。具有高转移潜能的MHCC-97H形成VM的能力强于低转移潜能的MHCC-97L和无转移潜能的Huh-7，L-02中未形成VM。RT-PCR和蛋白质印迹法的结果一致。
肝癌组织中MIG7表达较高，且与VM形成呈正相关。不同肝癌细胞系中MIG7的表达与其VM形成、侵袭和转移情况一致。MIG7是抑制肝癌侵袭和转移的潜在靶点。
