Research Group on Quality, Safety, and Bioactivity of Plant Foods, Laboratory of Food & Health, Department of Food Science and Technology, CEBAS-CSIC , 30100 Campus de Espinardo, Murcia Spain.
Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University , Ghent, Belgium.
J Agric Food Chem. 2017 Jul 12;65(27):5480-5493. doi: 10.1021/acs.jafc.7b02049. Epub 2017 Jun 28.
A TWIN-SHIME system was used to compare the metabolism of pomegranate polyphenols by the gut microbiota from two individuals with different urolithin metabotypes. Gut microbiota, ellagitannin metabolism, short-chain fatty acids (SCFA), transport of metabolites, and phase II metabolism using Caco-2 cells were explored. The simulation reproduced the in vivo metabolic profiles for each metabotype. The study shows for the first time that microbial composition, metabolism of ellagitannins, and SCFA differ between metabotypes and along the large intestine. The assay also showed that pomegranate phenolics preserved intestinal cell integrity. Pomegranate polyphenols enhanced urolithin and propionate production, as well as Akkermansia and Gordonibacter prevalence with the highest effect in the descending colon. The system provides an insight into the mechanisms of pomegranate polyphenol gut microbiota metabolism and absorption through intestinal cells. The results obtained by the combined SHIME/Caco-2 cell system are consistent with previous human and animal studies and show that although urolithin metabolites are present along the gastrointestinal tract due to enterohepatic circulation, they are predominantly produced in the distal colon region.
采用双 SHIME 系统比较了来自两种具有不同尿石素代谢表型个体的肠道微生物群对石榴多酚的代谢。研究了肠道微生物群、鞣花单宁代谢、短链脂肪酸 (SCFA)、代谢物转运以及使用 Caco-2 细胞的 II 相代谢。该模拟再现了每种代谢表型的体内代谢谱。该研究首次表明，微生物组成、鞣花单宁代谢和 SCFA 在代谢表型之间以及沿着大肠存在差异。该试验还表明，石榴多酚可保持肠道细胞完整性。石榴多酚增强了尿石素和丙酸的产生，以及 Akkermansia 和 Gordonibacter 的流行，在降结肠中效果最高。该系统深入了解了通过肠道细胞进行的石榴多酚肠道微生物群代谢和吸收的机制。通过组合 SHIME/Caco-2 细胞系统获得的结果与之前的人体和动物研究一致，并表明尽管由于肠肝循环尿石素代谢物存在于整个胃肠道中，但它们主要在远端结肠区域产生。
