Rosier Bas J H M, Cremers Glenn A O, Engelen Wouter, Merkx Maarten, Brunsveld Luc, de Greef Tom F A
Laboratory of Chemical Biology and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
Chem Commun (Camb). 2017 Jun 29;53(53):7393-7396. doi: 10.1039/c7cc04178k.
A photocrosslinkable protein G variant was used as an adapter protein to covalently and site-specifically conjugate an antibody and an Fc-fusion protein to an oligonucleotide. This modular approach enables straightforward decoration of DNA nanostructures with complex native proteins while retaining their innate binding affinity, allowing precise control over the nanoscale spatial organization of such proteins for in vitro and in vivo biomedical applications.
一种可光交联的蛋白G变体被用作衔接蛋白，将抗体和Fc融合蛋白共价且位点特异性地连接到寡核苷酸上。这种模块化方法能够用复杂的天然蛋白质直接修饰DNA纳米结构，同时保留其固有的结合亲和力，从而能够精确控制此类蛋白质在体外和体内生物医学应用中的纳米级空间组织。
