Department of Biochemistry, CHU de Caen, Caen, France.
Eur Rev Med Pharmacol Sci. 2017 May;21(10):2463-2466.
Troponins are considered as the biomarkers of choice to highlight cardiac injury in emergency departments, but are also valuable to detect cardiac injury in a non-emergency setting. In this latter case, transport of blood samples to laboratories often exceeds the manufacturer's recommendations (<2 h between vein puncture and analysis for the Beckman Coulter AccuTnI+3 assay). We aim to evaluate in vitro the stability of troponin Ic (cTnI) at two intervals (<2 h and at 4 h) over a wide range of concentrations using the Beckman Coulter AccuTnI+3 assay.
For each of the 95 patients included in this study, we analyzed the first blood sample with a time of transport <2 h, and the second sample after 4 h from vein puncture. We then calculated the correlation between the two periods of analysis and evaluated the bias by a Bland-Altman test.
Taking into account of our analytical reproducibility, we did not observe any significant differences in cTnI values between <2 h and 4 h.
The time between vein puncture and analysis of cTnI can be extended to 4 h.
肌钙蛋白被认为是急诊科心脏损伤的首选生物标志物，但在非紧急情况下也可用于检测心脏损伤。在后一种情况下，将血样运送到实验室通常会超过制造商的建议（贝克曼库尔特 AccuTnI+3 检测法要求在静脉穿刺和分析之间的时间间隔<2 小时）。我们旨在使用贝克曼库尔特 AccuTnI+3 检测法，在两个时间间隔（<2 小时和 4 小时）内，在广泛的浓度范围内，评估肌钙蛋白 Ic（cTnI）的体外稳定性。
对于本研究中纳入的 95 名患者中的每一位，我们分析了静脉穿刺后<2 小时内的第一份血样，以及 4 小时后的第二份血样。然后，我们计算了两个分析时间段之间的相关性，并通过 Bland-Altman 检验评估了偏差。
考虑到我们的分析可重复性，我们没有观察到<2 小时和 4 小时之间 cTnI 值有任何显著差异。
静脉穿刺和 cTnI 分析之间的时间可以延长至 4 小时。
