Jing S-Y, Jing S-Q, Liu L-L, Xu L-F, Zhang F, Gao J-L
Department of Neurosurgery, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan, China.
Eur Rev Med Pharmacol Sci. 2017 May;21(10):2421-2425.
MicroRNAs (miRNAs) are epigenetic regulators of gene expression, and their deregulation plays an important role in human cancer, including glioma. The main objective of this work was to investigate the expression level of miR-373 and its clinical significance in glioma.
The expression levels of miR-373 in glioma tissues and non-neoplastic brain tissues were measured by the qRT-PCR assay. Patients were divided into two groups based on the median miR-373 expression. The probability of differences in overall and progression-free survival as a function of time was ascertained by use of the Kaplan-Meier method. Cox regression analysis of factors potentially associated with survival was conducted to identify independent factors.
In clinical gastric cancer samples, we found that miR-373 expression was significantly down-regulated in glioma tissues compared with non-neoplastic brain tissues (p<0.01). Reduced expression of miR-373 was associated with serum WHO grade (p=0.015) and KPS score (p=0.001). Kaplan-Meier analysis indicated that patients with low level of miR-373 expression had poorer overall survival (OS) and progression-free survival (PFS). Multivariate survival analysis verified that miR-373 expression level was an independent predictor of both OS and PFS for glioma patients.
Our study showed miR-373 was associated to progression in glioma, and suggested it as a potential predictive factor for the prognosis of glioma.
微小RNA（miRNA）是基因表达的表观遗传调节因子，其失调在包括胶质瘤在内的人类癌症中起重要作用。本研究的主要目的是探讨miR-373在胶质瘤中的表达水平及其临床意义。
采用qRT-PCR检测胶质瘤组织和非肿瘤性脑组织中miR-373的表达水平。根据miR-373表达中位数将患者分为两组。采用Kaplan-Meier法确定总生存期和无进展生存期差异随时间变化的概率。对可能与生存相关的因素进行Cox回归分析以确定独立因素。
在临床胃癌样本中，我们发现与非肿瘤性脑组织相比，胶质瘤组织中miR-373表达显著下调（p<0.01）。miR-373表达降低与血清WHO分级（p=0.015）和KPS评分（p=0.001）相关。Kaplan-Meier分析表明，miR-373表达水平低的患者总生存期（OS）和无进展生存期（PFS）较差。多因素生存分析证实，miR-373表达水平是胶质瘤患者OS和PFS的独立预测因子。
我们的研究表明miR-373与胶质瘤进展相关，并提示其作为胶质瘤预后的潜在预测因子。
