Intra-splenic transplantation of allogenic isolated pancreatic islets in nonimmunosuppressed rats. A morphological immunocytochemical study.

Adult rat islets isolated by collagenase technique were injected into the spleen in 10 Alloxantreated allogenic Wistar rats. No immunosuppression was used. Recipients were examined on the 1st, 2nd, 3rd, 4th and 5th after transplantation. Localization of insulin, glucagon and somatostatin-immunoreactive cells were demonstrated in all implants from the 1st to the 5th d. There were considerable differences in topographical arrangement of glucagon- and somatostatin-immunoreactive cells in transplanted islets from the second day.