Arcella Antonietta, Oliva Maria Antonietta, Sanchez Massimo, Staffieri Sabrina, Esposito Vincenzo, Giangaspero Felice, Cantore Giampaolo
I.R.C.C.S. I.N.M. Neuromed, Pozzilli, Italy.
I.S.S., Rome.
Environ Toxicol. 2017 Sep;32(9):2113-2123. doi: 10.1002/tox.22419. Epub 2017 Jun 15.
Hispolon is a polyphenolic compound isolated from Phellinus linteus which exhibits antitumor activity. Here, we explored the effects of hispolon on human glioblastoma cells U87MG. Cell viability was examined by MTT assay. Growth was investigated by incubating cells with various concentrations of hispolon (25 and 50 µM) for 24, 48 or 72 h and daily cell count. Cell cycle and apoptosis assay were assessed by flow cytometry. Hispolon decreased cell viability in a dose- and time-dependent manner. The cell cycle distribution showed that hispolon enhanced the accumulation of the cells in G2/M phase. Hispolon decreased the expression of G1-S transition-related protein cyclin D4 but increased the expression of CDK inhibitor p21. Additionally, hispolon enhanced the expression of p53. Moreover, hispolon treatment was effective on U87MG cells in inhibiting cell viability and inducing cell apoptosis. Our results indicate that hispolon inhibits the cell viability, induces G2/M cell cycle arrest and apoptosis in glioblastoma U87MG cells, and p53 should play a role in hispolon-mediated antitumor activity.
桑黄多糖是从桑黄中分离出的一种具有抗肿瘤活性的多酚类化合物。在此，我们探讨了桑黄多糖对人胶质母细胞瘤U87MG细胞的影响。通过MTT法检测细胞活力。通过用不同浓度的桑黄多糖（25和50 μM）处理细胞24、48或72小时并每日进行细胞计数来研究细胞生长情况。通过流式细胞术评估细胞周期和凋亡检测。桑黄多糖以剂量和时间依赖性方式降低细胞活力。细胞周期分布表明，桑黄多糖增强了细胞在G2/M期的积累。桑黄多糖降低了G1-S转换相关蛋白细胞周期蛋白D4的表达，但增加了细胞周期蛋白依赖性激酶抑制剂p21的表达。此外，桑黄多糖增强了p53的表达。而且，桑黄多糖处理对U87MG细胞抑制细胞活力和诱导细胞凋亡有效。我们的结果表明，桑黄多糖抑制胶质母细胞瘤U87MG细胞的细胞活力，诱导G2/M期细胞周期阻滞和凋亡，并且p53在桑黄多糖介导的抗肿瘤活性中发挥作用。
