Department of Anatomy, Tokyo Dental College, 2-9-18 Misaki-cho, Tokyo, Japan.
Department of Anatomy, Tokyo Dental College, 2-9-18 Misaki-cho, Tokyo, Japan.
Arch Oral Biol. 2017 Oct;82:71-78. doi: 10.1016/j.archoralbio.2017.06.001. Epub 2017 Jun 6.
This study was performed to investigate how the palatine aponeurosis, medial pterygoid process (MPP) of the sphenoid bone, and tensor veli palatini (TVP) muscle form the pulley: muscle-tendon-bone complex.
Mice at embryonic day (ED) 14-17 were used as sample in this study. Azan staining was performed to observe the morphology, and immunohistochemical staining of desmin was performed to closely observe the development of the myotendinous junction. To confirm the bone formation process, immunohistochemical staining of type II collagen (col II), tartrate-resistant acid phosphatase (TRAP), and alkaline phosphatase (ALP) staining were performed. Furthermore, to objectively evaluate bone formation, the major axis and width of the MPP were measured, and osteoclasts that appeared in the MPP were counted.
At ED 14 and 14.5, ALP showed a reaction throughout the MPP. The col II-positive area expanded until ED 16.5, but it was markedly reduced at ED 17. The TVP initially contacted with the palatine aponeurosis at ED 16.5. The major axis and width of the MPP and the number of TRAP-positive osteoclasts were significantly increased as the TVP and palatine aponeurosis joined.
Therefore, in addition to the tissue units: muscle, tendon, and bone, the interaction in organogenesis promotes rapid growth of the pulley: muscle-tendon-bone complex.
本研究旨在探讨腭帆张肌、蝶骨翼内板和腭帆提肌如何形成滑轮：肌-腱-骨复合体。
本研究以胚胎期 14-17 天的小鼠为样本。进行阿赞染色观察形态学，免疫组织化学染色观察结蛋白发育，以紧密观察肌-腱结合部的发育。为了证实骨形成过程，进行 II 型胶原（col II）、抗酒石酸酸性磷酸酶（TRAP）和碱性磷酸酶（ALP）染色的免疫组织化学染色。此外，为了客观评估骨形成，测量翼内板的长轴和宽度，并计数翼内板中的破骨细胞。
在 ED 14 和 14.5 时，ALP 在整个翼内板中呈反应性。col II 阳性区域扩大到 ED 16.5，但在 ED 17 时明显减少。TVP 最初在 ED 16.5 与腭帆张肌接触。随着 TVP 和腭帆张肌的结合，翼内板的长轴和宽度以及 TRAP 阳性破骨细胞的数量显著增加。
因此，除了组织单位：肌肉、肌腱和骨骼之外，器官发生中的相互作用促进了滑轮：肌-腱-骨复合体的快速生长。
