[Expression of gamma-glutamyl transpeptidase in the IAR 2 cell line cultured on a nonadhesive substrate].

Expression of gamma-glutamyltranspeptidase (GGT) in liver epithelial IAR 2 cells was studied after culturing on adhesive and non-adhesive substrates. IAR 2 cells are non-tumorigenic and do not express GGT under normalcy. Culturing these cells on a non-adhesive substrate dramatically retards the normal spreading up of these cells. Individual "islets" of the cells begin to express GGT activity tested by histochemistry. Biochemical testing of GGT activity in IAR 2 cells cultured on adhesive and non-adhesive substrates confirmed an assumption that maximal expression of GGT coincides in time with maximal morphological differences in the cells cultured on these substrates.