Gujjala Sudhakara, Putakala Mallaiah, Nukala Srinivasulu, Bangeppagari Manjunatha, Rajendran Ramaswamy, Desireddy Saralakumari
Dept. of Biochemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh, 515003, India.
Center for Biofluid and Biomimic Research, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.
Biomed Pharmacother. 2017 Aug;92:1062-1072. doi: 10.1016/j.biopha.2017.06.016. Epub 2017 Jun 10.
The present study was aimed to evaluate the modulatory effects of hydroalcoholic extract of Caralluma fimbriata (CFE) by assaying the activities of key enzymes of carbohydrate metabolism and changes in glycogen content (liver and muscle) in high-fat (HF) diet-induced diabetic rats. In vitro glucose uptake studies were carried out in both psoas muscle and adipose tissue. The inhibitory effect of the extract on α-amylase was determined in in vitro studies. Male Wistar rats of body weight around 180g were divided into five groups (n=8), two of these groups were fed with standard pellet diet and the other three groups were fed with HF- (60%) diet. CFE (200mg/kg body weight/day) was administered through oral route to each group of standard pellet diet rats and HF-fed rats and Metformin (Met) (20mg/kg body weight/day) was administered through oral route to HFD+Met group for 90 days. At the end of the experimental period, biochemical parameters related to glycogen content in liver and muscle, and intestinal disaccharidases like maltase, sucrase and lactase were assayed. Alterations in the activities of enzymes of glucose metabolism (hexokinase, phosphorfructoki nase, pyruvate kinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, and glucose-6-phosphate dehydrogenase), intestinal disaccharidases and glycogen content as observed in the high fat diet-fed rats were prevented with CFE/Met administration. From this study, we observed that CFE/Met could significantly restore the levels of glycogen in liver and muscle and key enzymes of carbohydrate metabolism to near normal in groups-HFD+CFE and HFD+Met. The skeletal muscle of HF-diet fed rats showed degenerative changes of muscle myofibers with fat deposition. These changes were attenuated in the HFD group treated with CFE/Met and retained their normal structure appearance. It can be concluded from these results that CFE might be of value in reducing the alterations related to carbohydrate metabolism under high calorie diet consumption.
本研究旨在通过测定高脂（HF）饮食诱导的糖尿病大鼠碳水化合物代谢关键酶的活性以及糖原含量（肝脏和肌肉）的变化，评估肉苁蓉水醇提取物（CFE）的调节作用。在腰大肌和脂肪组织中进行了体外葡萄糖摄取研究。在体外研究中测定了提取物对α-淀粉酶的抑制作用。将体重约180g的雄性Wistar大鼠分为五组（n = 8），其中两组喂食标准颗粒饲料，另外三组喂食HF-（60%）饲料。将CFE（200mg/kg体重/天）经口给予每组标准颗粒饲料大鼠和HF喂养大鼠，将二甲双胍（Met）（20mg/kg体重/天）经口给予HFD + Met组，持续90天。在实验期结束时，测定了与肝脏和肌肉中糖原含量以及肠双糖酶（如麦芽糖酶、蔗糖酶和乳糖酶）相关的生化参数。CFE/Met给药可预防高脂饮食喂养大鼠中观察到的葡萄糖代谢酶（己糖激酶、磷酸果糖激酶、丙酮酸激酶、葡萄糖-6-磷酸酶、果糖-1,6-二磷酸酶和葡萄糖-6-磷酸脱氢酶）、肠双糖酶和糖原含量的活性变化。从本研究中，我们观察到CFE/Met可使HFD + CFE组和HFD + Met组肝脏和肌肉中的糖原水平以及碳水化合物代谢关键酶显著恢复至接近正常水平。HF饮食喂养大鼠的骨骼肌显示出肌纤维的退行性变化并伴有脂肪沉积。在接受CFE/Met治疗的HFD组中，这些变化得到减轻并保留了其正常的结构外观。从这些结果可以得出结论，CFE在减少高热量饮食消耗下与碳水化合物代谢相关的改变方面可能具有价值。
