Department of Virology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
Turk J Med Sci. 2017 Jun 12;47(3):960-966. doi: 10.3906/sag-1509-87.
BACKGROUND/AIM: There are limited studies on genotyping and phylogenetic analysis of norovirus in Turkey, and this has not previously been studied in the Eastern Anatolia region. The aim of the present study was to determine the norovirus profile in this region with genotyping and phylogenetic analysis.
Included in the study were stool samples obtained from 427 people from different age groups in Eastern Anatolia. The nucleic acid samples isolated by the automatic system and nucleic acid sequence reactions and phylogenetic analyses were performed on RNA samples.
The presence of norovirus was detected in 86 (20.1%) of the 427 stool samples by RT-PCR analysis. Twenty-six samples selected randomly from norovirus-RNA positive samples were subjected to the sequence reaction. In 24 of the 26 samples, genogroup GII was determined, as well as one each from GI and GIV in sequence reactions. Four different genotypes were detected in genogroup GII, which were determined to be the dominant types. These were GII.1, GII.4, GII.16, and GII.21. The GI.6 and GIV.1 genotypes were determined in genogroups GI and GIV, respectively.
The high frequency and genetic diversity of these infections are risk factors for disease and so vaccine studies should be undertaken in consideration of this situation.
背景/目的：在土耳其，有关诺如病毒的基因分型和系统发育分析的研究有限，而在东安纳托利亚地区，这方面的研究尚未开展。本研究的目的是通过基因分型和系统发育分析，确定该地区的诺如病毒特征。
本研究纳入了来自东安纳托利亚不同年龄组的 427 人粪便样本。通过自动系统分离核酸样本，并对 RNA 样本进行核酸序列反应和系统发育分析。
通过 RT-PCR 分析，在 427 份粪便样本中检测到 86 份（20.1%）诺如病毒阳性。从诺如病毒 RNA 阳性样本中随机选择 26 份进行序列反应。在 26 个样本中，有 24 个确定为基因 GII，序列反应中还分别确定了 1 个 GI 和 GIV。在基因 GII 中检测到 4 种不同的基因型，它们被确定为优势类型。这些分别是 GII.1、GII.4、GII.16 和 GII.21。在基因 GII 和 GIV 中分别确定了 GI.6 和 GIV.1 基因型。
这些感染的高频率和遗传多样性是疾病的危险因素，因此应考虑这种情况开展疫苗研究。
