土耳其伊斯坦布尔地区腹泻儿童中诺如病毒的流行情况及系统发育分析。

Frequency and phylogeny of norovirus in diarrheic children in Istanbul, Turkey.

机构信息

Başkent University Hospital, Clinic of Child Health and Diseases, Altunizade, Istanbul, Turkey.

出版信息

J Clin Virol. 2011 Jul;51(3):160-4. doi: 10.1016/j.jcv.2011.03.004. Epub 2011 May 17.

DOI:10.1016/j.jcv.2011.03.004

PMID:21592853

Abstract

BACKGROUND

Norovirus (NoV) is recognised as one of the most common causes of foodborne infections. Contaminated shellfish, food, water and hospitals are well documented sources of the virus.

OBJECTIVE

NoV in diarrheic children has not previously been investigated in Istanbul, Turkey, hence the aim of this study was to detect and investigate the frequency and phylogeny of human NoV genogroups I and II in children with acute gastroenteritis.

STUDY DESIGN

238 stool samples were collected from diarrheic children from 2 hospitals (Cerrahpasa Medical School and Haseki) in Istanbul and analysed by ELISA, RT-PCR and real-time RT-PCR using both SYBR Green and probe-based assays for human NoV. Primers targeting the RNA-polymerase gene were used for RT-PCR to allow DNA sequencing of Turkish NoV strains and phylogenetic analysis to be performed.

RESULTS

NoV GII was detected in 36 (15.1%) of 238 samples by SYBR Green real-time RT-PCR, 10.9% by a probe-based real-time RT-PCR and 10.5% by ELISA (Ridascreen). Genogroup II (GII) the Turkish NoVs clustered with including GII4 (72.2%), GII16 (5.5%), GIIb (16.7%) and GIIe (5.5%). Two variants of GII4 (GII4-2006b and GII4-2008), GII16 and recombinant noroviruses (GIIb and GIIe) were identified.

CONCLUSION

This study shows a high frequency and genetic diversity of NoV GII infections in children with acute gastroenteritis in Istanbul, Turkey.

摘要

背景

诺如病毒（NoV）是食源性感染的最常见原因之一。受污染的贝类、食物、水和医院是该病毒的已知来源。

目的

此前，土耳其伊斯坦布尔尚未对腹泻儿童中的诺如病毒进行研究，因此本研究旨在检测和调查急性胃肠炎患儿中人类 NoV 基因 I 和 II 型的频率和系统发育。

研究设计

从伊斯坦布尔的两家医院（Cerrahpasa 医学院和 Haseki）采集了 238 份腹泻儿童的粪便样本，通过 ELISA、RT-PCR 和实时 RT-PCR 用 SYBR Green 和探针检测试剂盒检测人类 NoV。用于 RT-PCR 的 RNA 聚合酶基因引物允许对土耳其 NoV 株进行 DNA 测序和系统发育分析。

结果

SYBR Green 实时 RT-PCR 检测到 36 份（15.1%）238 份样本中存在 NoV GII，探针实时 RT-PCR 检测到 10.9%，ELISA（Ridascreen）检测到 10.5%。基因型 II（GII）的土耳其 NoVs 与包括 GII4（72.2%）、GII16（5.5%）、GIIb（16.7%）和 GIIe（5.5%）的病毒聚类在一起。鉴定出两种 GII4（GII4-2006b 和 GII4-2008）、GII16 和重组诺如病毒（GIIb 和 GIIe）变体。