Calonghi N, Parolin C, Sartor G, Verardi L, Giordani B, Frisco G, Marangoni A, Vitali B
1 Department of Pharmacy and Biotechnology, University of Bologna, Via San Donato 15, 40127 Bologna, Italy.
2 Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy.
Benef Microbes. 2017 Aug 24;8(4):625-633. doi: 10.3920/BM2016.0212. Epub 2017 Jun 16.
Vaginal lactobacilli offer protection against recurrent urinary and vaginal infections. The precise mechanisms underlying the interaction between lactobacilli and the host epithelium remain poorly understood at the molecular level. Deciphering such events can provide valuable information on the mode of action of commensal and probiotic bacteria in the vaginal environment. We investigated the effects exerted by five Lactobacillus strains of vaginal origin (Lactobacillus crispatus BC1 and BC2, Lactobacillus gasseri BC9 and BC11 and Lactobacillus vaginalis BC15) on the physical properties of the plasma membrane in a cervical cell line (HeLa). The interaction of the vaginal lactobacilli with the cervical cells determined two kinds of effects on plasma membrane: (1) modification of the membrane polar lipid organisation and the physical properties (L. crispatus BC1 and L. gasseri BC9); (2) modification of α5β1 integrin organisation (L. crispatus BC2, L. gasseri BC11 and L. vaginalis BC15). These two mechanisms can be at the basis of the protective role of lactobacilli against Candida albicans adhesion. Upon stimulation with all Lactobacillus strains, we observed a reduction of the basal oxidative stress in HeLa cells that could be related to modifications in physical properties and organisation of the plasma membrane. These results confirm the strictly strain-specific peculiarities of Lactobacillus and deepen the understanding of the mechanisms underlying the health-promoting role of this genus within the vaginal ecosystem.
阴道乳酸杆菌可预防复发性泌尿和阴道感染。在分子水平上，乳酸杆菌与宿主上皮细胞之间相互作用的精确机制仍知之甚少。解读这些事件可为阴道环境中共生菌和益生菌的作用模式提供有价值的信息。我们研究了五株阴道来源的乳酸杆菌菌株（卷曲乳酸杆菌BC1和BC2、加氏乳酸杆菌BC9和BC11以及阴道乳酸杆菌BC15）对宫颈细胞系（HeLa）质膜物理性质的影响。阴道乳酸杆菌与宫颈细胞的相互作用对质膜产生了两种影响：（1）膜极性脂质组织和物理性质的改变（卷曲乳酸杆菌BC1和加氏乳酸杆菌BC9）；（2）α5β1整合素组织的改变（卷曲乳酸杆菌BC2、加氏乳酸杆菌BC11和阴道乳酸杆菌BC15）。这两种机制可能是乳酸杆菌对白色念珠菌黏附具有保护作用的基础。在用所有乳酸杆菌菌株刺激后，我们观察到HeLa细胞中基础氧化应激的降低，这可能与质膜物理性质和组织的改变有关。这些结果证实了乳酸杆菌严格的菌株特异性特性，并加深了对该属在阴道生态系统中促进健康作用机制的理解。
