Elshafei Ahmed, Shaker Olfat, Abd El-Motaal Ossama, Salman Tarek
1 Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
2 Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
Tumour Biol. 2017 Jun;39(6):1010428317705765. doi: 10.1177/1010428317705765.
Dysregulation in microRNA expression is a common feature in colorectal cancer. Due to the inconsistent results regarding serum miR-92a expression pattern and the insufficient studies on serum miR-375 and miR-760, we aimed in this study to investigate their expression profile and diagnostic and prognostic power in Egyptian colorectal cancer patients. The expression profile of miR-92a, miR-375, and miR-760 was determined in the sera of 64 colorectal cancer patients using quantitative real-time reverse transcription polymerase chain reaction in comparison to 27 healthy control subjects. The expression fold change of the studied microRNAs was correlated with patients' clinicopathological features. Receiver operating characteristic curve analysis was done to determine the role of these microRNAs in colorectal cancer diagnosis and follow-up according to the yielded area under the curve. The expression pattern of miR-92a was significantly upregulated (3.38 ± 2.52, p < 0.0001), while both of miR-375 and 760 were significantly downregulated (-1.250 ± 1.80, p< 0.0001; -1.710 ± 1.88, p < 0.0001, respectively) in colorectal cancer than the control. MiR-92a was positively correlated ( r = 0.671, p = 0.0001), while miR-375 and miR-760 were inversely correlated ( r = -0.414, p = 0.001; r = -0.644, p = 0.0001) with advanced colorectal cancer stages. Receiver operating characteristic curve analysis disclosed the highest diagnostic potential for miR-760 to discriminate colorectal cancer patients and early-stage colorectal cancer from the control (area under the curve = 0.922 and 0.875, respectively), while the highest prognostic potential for discrimination between colorectal cancer stages was for miR-92a. In conclusion, serum level of miR-92a, miR-375, and miR-760 may serve as biomarkers of colorectal cancer in Egyptian patients with high diagnostic power for miR-760 and high prognostic power for miR-92a.
微小RNA表达失调是结直肠癌的一个常见特征。由于血清miR-92a表达模式的结果不一致，以及对血清miR-375和miR-760的研究不足，我们在本研究中旨在调查它们在埃及结直肠癌患者中的表达谱以及诊断和预后能力。与27名健康对照受试者相比，使用定量实时逆转录聚合酶链反应测定了64名结直肠癌患者血清中miR-92a、miR-375和miR-760的表达谱。所研究的微小RNA的表达倍数变化与患者的临床病理特征相关。进行了受试者操作特征曲线分析，以根据曲线下面积确定这些微小RNA在结直肠癌诊断和随访中的作用。与对照组相比，结直肠癌中miR-92a的表达模式显著上调（3.38±2.52，p<0.0001），而miR-375和760均显著下调（分别为-1.250±1.80，p<0.0001；-1.710±1.88，p<0.0001）。miR-92a呈正相关（r=0.671，p=0.0001），而miR-375和miR-760与晚期结直肠癌分期呈负相关（r=-0.414，p=0.001；r=-0.644，p=0.0001）。受试者操作特征曲线分析显示，miR-760区分结直肠癌患者和早期结直肠癌与对照组的诊断潜力最高（曲线下面积分别为0.922和0.875），而区分结直肠癌分期的预后潜力最高的是miR-92a。总之，miR-92a、miR-375和miR-760的血清水平可能作为埃及患者结直肠癌的生物标志物，其中miR-760具有高诊断能力，miR-92a具有高预后能力。
