Department of Oncology, Cangzhou Central Hospital, Cangzhou, Hebei, China.
J Cell Mol Med. 2020 Aug;24(15):8363-8367. doi: 10.1111/jcmm.15282. Epub 2020 Jun 20.
Colorectal cancer (CRC) is one of the most common cancers worldwide, with high mortality. Abnormally expressed microRNAs (miRNAs) are considered novel biomarkers in cancer diagnosis. The aim of this study was to investigate the diagnostic value of miR-92a-1 in patients with CRC. Serum samples were collected from 148 patients pathologically diagnosed with CRC and 68 gender- and age-matched healthy volunteers. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure serum miR-92a-1 level. Relationship between miR-92a-1 and clinicopathological features of CRC cases was analysed via chi-square test. Receiver operating characteristic (ROC) curve was plotted to estimate the diagnostic value of miR-92a-1 in CRC. Serum miR-92a-1 was significantly up-regulated in CRC patients compared with healthy individuals (P < .001). Moreover, miR-92a-1 expression was correlated with TNM stage (P = .02), histological stage (P = .003), lymph node metastasis (P = .003) and distant metastasis (P < .001). ROC analysis showed that the area under the ROC curve (AUC) was 0.914, suggesting high diagnostic accuracy of miR-92a-1 in ROC. The optimal cut-off value was 1.485, with a sensitivity of 81.8% and a specificity of 95.6%. MiR-92a-1 is increased in CRC patients and correlated with aggressive clinical characteristics. Serum miR-92a-1 may be a potential diagnostic biomarker for CRC.
结直肠癌（CRC）是全球最常见的癌症之一，死亡率较高。异常表达的 microRNAs（miRNAs）被认为是癌症诊断的新型生物标志物。本研究旨在探讨 miR-92a-1 在 CRC 患者中的诊断价值。收集了 148 例经病理诊断为 CRC 的患者和 68 名性别和年龄匹配的健康志愿者的血清样本。采用实时定量聚合酶链反应（qRT-PCR）检测血清 miR-92a-1 水平。通过卡方检验分析 miR-92a-1 与 CRC 病例临床病理特征的关系。绘制受试者工作特征（ROC）曲线评估 miR-92a-1 对 CRC 的诊断价值。与健康个体相比，CRC 患者血清 miR-92a-1 水平显著上调（P<0.001）。此外，miR-92a-1 的表达与 TNM 分期（P=0.02）、组织学分期（P=0.003）、淋巴结转移（P=0.003）和远处转移（P<0.001）相关。ROC 分析显示，ROC 曲线下面积（AUC）为 0.914，提示 miR-92a-1 对 CRC 的诊断准确性较高。最佳截断值为 1.485，灵敏度为 81.8%，特异性为 95.6%。miR-92a-1 在 CRC 患者中升高，与侵袭性临床特征相关。血清 miR-92a-1 可能是 CRC 的潜在诊断生物标志物。
