Del Carmen Baez Maria, Taran Mariana, de La Paz Scribano Maria, Balceda Ariel, Buonanotte Carla, Blencio Sergio, Fonseca Ismael, Moya Monica
Catedra de Fisica Biomedica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, X5000 Cordoba. Argentina.
II Catedra de Anatomia Patologica, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, X5000 Cordoba. Argentina.
Antiinflamm Antiallergy Agents Med Chem. 2017;16(2):87-93. doi: 10.2174/1871523016666170616121133.
The oxidative process in atherogenesis generated by proinflammatory induction and response to antioxidants vitamins in an experimental model were analyzed.
Male rats were used: (A)Control, (B)Control+vitamin E plus C, (C)Hyperfibrinogenemia and (D)Hyperfibrinogenemia+vitamins E plus C. Hyperfibrinogenemia induced by daily injection of adrenaline (0.1mg/day/rat) for 120 days.
3.42 mg/kg of vitamin E plus 2.14 mg/kg of vitamin C, fifteen days after induction. Vascular histology analyzed by optical microscopy. Fibrinogen, nitrites and superoxide dismutase analyzed by spectrophotometry.
MANOVA, Hotelling test for post testing, significance level p<0.05.
(C) group showed higher fibrinogen than (A) and (B)(p<0.001). Compared to (C) group, (D) showed a decrease of fibrinogen (p<0.001). A marked increase in nitrites was found in (C) versus (A), (B) and (D) groups (p<0.001). Superoxide dismutase activity increased in (C) group compared to groups (A) and (B) (p<0.001). In the group (D) an increase of the activity of this enzyme was observed in comparison to groups (C)(p<0.001), (A) and (B) (p<0.0001 in both). The (C) group shown endothelial denudation, thickening of the vascular intima and extracellular matrix enlargement with foam cells(p<0.001).
These results strongly suggest that vitamins E plus C produce regression of inflammatory and oxidative stress processes in this experimental model.
分析在一个实验模型中，由促炎诱导产生的动脉粥样硬化形成过程中的氧化过程以及对抗氧化维生素的反应。
使用雄性大鼠：(A)对照组，(B)对照组+维生素E加C，(C)高纤维蛋白原血症组，(D)高纤维蛋白原血症+维生素E加C组。通过每天注射肾上腺素（0.1毫克/天/只大鼠）持续120天诱导高纤维蛋白原血症。
诱导15天后，给予3.42毫克/千克的维生素E加2.14毫克/千克的维生素C。通过光学显微镜分析血管组织学。通过分光光度法分析纤维蛋白原、亚硝酸盐和超氧化物歧化酶。
多变量方差分析，采用Hotelling检验进行事后检验，显著性水平p<0.05。
(C)组的纤维蛋白原水平高于(A)组和(B)组（p<0.001）。与(C)组相比，(D)组的纤维蛋白原水平降低（p<0.001）。与(A)组、(B)组和(D)组相比，(C)组的亚硝酸盐显著增加（p<0.001）。与(A)组和(B)组相比，(C)组的超氧化物歧化酶活性增加（p<0.001）。与(C)组相比，(D)组该酶的活性增加（p<0.001），与(A)组和(B)组相比也增加（两者均为p<0.0001）。(C)组出现内皮剥脱、血管内膜增厚以及细胞外基质增大并伴有泡沫细胞（p<0.001）。
这些结果强烈表明，维生素E加C在该实验模型中可使炎症和氧化应激过程发生消退。
