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胆甾醇生物合成:不同柳珊瑚黄藻成分中角鲨烯形成的定位

Gorgosterol biosynthesis: localization of squalene formation in the zooxanthellar component of various gorgonians.

作者信息

Anderson D G

出版信息

Comp Biochem Physiol B. 1985;81(2):423-8. doi: 10.1016/0305-0491(85)90336-0.

DOI:10.1016/0305-0491(85)90336-0
PMID:2861942
Abstract

Four species of gorgonians: three related pseudoplexaurids Pseudoplexaura porosa, P. flagellosa and P. wagenaari; and the unrelated Pseudopterogorgia americana, are sources of zooxanthellae capable, in purified broken cell preparations, of converting [14C]labeled farnesyl pyrophosphate into squalene. More extensive studies with P. porosa and P. americana zooxanthellae preparations characterize the conversion as enzymatic and demonstrate farnesyl pyrophosphate and reduced pyridine nucleotide as substrates. NADPH and NADH are essentially equivalent. Anaerobic conditions are not required. Despite numerous attempts zooxanthellae formation of sterols, including gorgosterol, from radioactive substrates was unsuccessful. By enzyme studies, we can show only the conversion of mevalonate into squalene as a zooxanthellae capability.

摘要

四种柳珊瑚

三种近缘的拟丛柳珊瑚属的多孔拟丛柳珊瑚、鞭状拟丛柳珊瑚和瓦氏拟丛柳珊瑚;以及不同属的美洲拟软柳珊瑚,是虫黄藻的来源,在纯化的破碎细胞制剂中,这些虫黄藻能够将[14C]标记的法呢基焦磷酸转化为角鲨烯。对多孔拟丛柳珊瑚和美洲拟软柳珊瑚的虫黄藻制剂进行的更广泛研究表明,这种转化是酶促反应,并证明法呢基焦磷酸和还原型吡啶核苷酸是底物。NADPH和NADH基本等效。不需要厌氧条件。尽管进行了多次尝试,但从放射性底物形成包括戈尔戈甾醇在内的甾醇的虫黄藻实验均未成功。通过酶学研究,我们只能证明甲羟戊酸转化为角鲨烯是虫黄藻的一种能力。

相似文献

1
Gorgosterol biosynthesis: localization of squalene formation in the zooxanthellar component of various gorgonians.胆甾醇生物合成:不同柳珊瑚黄藻成分中角鲨烯形成的定位
Comp Biochem Physiol B. 1985;81(2):423-8. doi: 10.1016/0305-0491(85)90336-0.
2
In vitro assay of squalene epoxidase of Saccharomyces cerevisiae.酿酒酵母鲨烯环氧酶的体外测定
Biochem Biophys Res Commun. 1987 Sep 15;147(2):556-64. doi: 10.1016/0006-291x(87)90967-3.
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Squalene synthetase.角鲨烯合酶
Mol Cell Biochem. 1979 Oct 15;27(2):97-116. doi: 10.1007/BF00218354.
4
Studies on the biosynthesis of cholesterol. XV. Mechanism of squalene biosynthesis from farnesyl pyrophosphate and from mevalonate.胆固醇生物合成的研究。十五、由法呢基焦磷酸酯及甲羟戊酸合成角鲨烯的机制。
J Biol Chem. 1961 Jul;236:1934-47.
5
Purification to homogeneity and some properties of squalene synthetase.角鲨烯合成酶的纯化至均一性及其某些性质
Arch Biochem Biophys. 1988 Feb 1;260(2):622-7. doi: 10.1016/0003-9861(88)90490-0.
6
Mechanism of squalene biosynthesis from mevalonate and farnesyl pyrophosphate.由甲羟戊酸和法呢基焦磷酸生物合成角鲨烯的机制。
Biochem Biophys Res Commun. 1961 Feb 24;4:138-42. doi: 10.1016/0006-291x(61)90363-1.
7
STEREOCHEMISTRY AT THE CENTER OF SQUALENE DURING ITS BIOSYNTHESIS FROM FARNESYL PYROPHOSPHATE AND SUBSEQUENT CONVERSION TO CHOLESTEROL.在焦磷酸法呢酯生物合成角鲨烯以及随后将其转化为胆固醇的过程中,角鲨烯中心的立体化学。
J Biol Chem. 1964 Jan;239:98-101.
8
Studies on the biosynthesis of cholesterol. XVI. Chemical synthesis of 1-H2-3-2-C-14- and 1-D2-2-C-14-trans-trans-farnesyl pyrophosphate and their utilization in squalene biosynthesis.胆固醇生物合成的研究。十六。1-H₂-3-²C-¹⁴-和1-D₂-2-²C-¹⁴-反式-反式-法呢基焦磷酸的化学合成及其在角鲨烯生物合成中的应用。
J Biol Chem. 1962 Jan;237:56-61.
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Stereochemistry of the hydrogen transfer to squalene during its biosynthesis from farnesyl pyrophosphate.在焦磷酸法呢酯生物合成角鲨烯过程中氢转移的立体化学。
Biochem Biophys Res Commun. 1963 Apr 23;11:125-8. doi: 10.1016/0006-291x(63)90078-0.
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Squalene synthetase. Solubilization and partial purification of squalene synthetase, copurification of presqualene pyrophosphate and squalene synthetase activities.角鲨烯合酶。角鲨烯合酶的溶解与部分纯化,前角鲨烯焦磷酸与角鲨烯合酶活性的共纯化。
J Biol Chem. 1987 Feb 5;262(4):1505-9.

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