Kaipainen P, Koivusaari U, Lang M
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;81(2):293-6. doi: 10.1016/0742-8413(85)90008-8.
Coumarin 7-hydroxylation activities were determined in the liver microsomes of eight different species: pig, mouse (three strains), rat, hamster, guinea-pig, rabbit, rainbow trout and crayfish. A high coumarin 7-hydroxylase activity was found in the microsomes of D2 mouse, rabbit, hamster and pig, an intermediate activity in case of B6 and AKR mice, rat and guinea-pig and a very low activity in rainbow trout and crayfish. In Ouchterlony immunodiffusion analysis our antibody against coumarin 7-hydroxylase specific cytochrome P-450 from D2 mice was able to form a weak precipitin line only with rat and hamster microsomes in addition to the three strains of mice (D2, B6, AKR) where the band was very sharp. Strongest inhibition by the antibody (50% or more) of microsomal coumarin 7-hydroxylase was found in the case of the three strains of mice and rabbit. In the case of the other species the inhibition was very weak or did not exist.
在猪、小鼠（三个品系）、大鼠、仓鼠、豚鼠、兔子、虹鳟鱼和小龙虾这八个不同物种的肝脏微粒体中测定了香豆素7-羟化活性。在D2小鼠、兔子、仓鼠和猪的微粒体中发现了较高的香豆素7-羟化酶活性，在B6和AKR小鼠、大鼠和豚鼠中活性中等，而在虹鳟鱼和小龙虾中活性非常低。在双向免疫扩散分析中，我们针对来自D2小鼠的香豆素7-羟化酶特异性细胞色素P-450的抗体，除了在三条带非常清晰的小鼠品系（D2、B6、AKR）外，仅与大鼠和仓鼠微粒体形成一条微弱的沉淀线。在三种小鼠品系和兔子中，抗体对微粒体香豆素7-羟化酶的抑制作用最强（50%或更高）。在其他物种中，抑制作用非常微弱或不存在。
