Ramírez-Miquet Evelio E, Cabrera Humberto, Grassi Hilda C, de J Andrades Efrén, Otero Isabel, Rodríguez Dania, Darias Juan G
Centro de Aplicaciones Tecnológicas y Desarrollo Nuclear (CEADEN), Calle 30 No. 502, Miramar C.P., 11300, La Habana, Cuba.
Laboratoire Matériaux et Phénomènes Quantiques, Université Paris Diderot, CNRS UMR 7162, Sorbonne Paris-Cité, 10 rue Alice Domon et Léonie Duquet, Paris, 75013, France.
Lasers Med Sci. 2017 Aug;32(6):1375-1386. doi: 10.1007/s10103-017-2256-0. Epub 2017 Jun 15.
This paper reports on the biospeckle processing of biological activity using a visualization scheme based upon the digital imaging information technology. Activity relative to bacterial growth in agar plates and to parasites affected by a drug is monitored via the speckle patterns generated by a coherent source incident on the microorganisms. We present experimental results to demonstrate the potential application of this methodology for following the activity in time. The digital imaging information technology is an alternative visualization enabling the study of speckle dynamics, which is correlated to the activity of bacteria and parasites. In this method, the changes in Red-Green-Blue (RGB) color component density are considered as markers of the growth of bacteria and parasites motility in presence of a drug. The RGB data was used to generate a two-dimensional surface plot allowing an analysis of color distribution on the speckle images. The proposed visualization is compared to the outcomes of the generalized differences and the temporal difference. A quantification of the activity is performed using a parameterization of the temporal difference method. The adopted digital image processing technique has been found suitable to monitor motility and morphological changes in the bacterial population over time and to detect and distinguish a short term drug action on parasites.
本文报道了一种基于数字成像信息技术的可视化方案对生物活性进行生物散斑处理的研究。通过相干光源照射微生物产生的散斑图案，监测琼脂平板中细菌生长以及受药物影响的寄生虫的相关活性。我们展示了实验结果，以证明该方法在实时跟踪活性方面的潜在应用。数字成像信息技术是一种用于研究散斑动态的替代可视化方法，散斑动态与细菌和寄生虫的活性相关。在该方法中，红绿蓝（RGB）颜色分量密度的变化被视为药物存在时细菌生长和寄生虫运动性的标志。RGB数据用于生成二维表面图，以便分析散斑图像上的颜色分布。将所提出的可视化方法与广义差分法和时间差分法的结果进行了比较。使用时间差分法的参数化对活性进行了量化。已发现所采用的数字图像处理技术适用于随时间监测细菌群体的运动性和形态变化，以及检测和区分药物对寄生虫的短期作用。
