动态激光散斑监测黑素瘤细胞中药物的作用。

Monitoring of the action of drugs in melanoma cells by dynamic laser speckle.

机构信息

Universidad de Valencia, Unidad de Biofísica y Física Médica, Departamento de Fisiología, Facultad de Medicina y Odontología, Valencia, Spain, CP 46010.

Universidade Federal de Lavras, Departamento de Engenharia, LavrasMG, Brazil, CP 3037.

出版信息

J Biomed Opt. 2014 May;19(5):057008. doi: 10.1117/1.JBO.19.5.057008.

DOI:10.1117/1.JBO.19.5.057008

PMID:24828071

Abstract

This work presents the development of a protocol based on the dynamic laser speckle designed to monitor the reaction of cancer cells of line MEL-RC08 to the application of the drug Colcemid in two different concentrations: 0.2 and 0.4 μg/mL. The protocol was designed using the forward scattering approach with an He-Ne laser of 632.8 nm illuminating the samples, a control, and two variations of Colcemid, being monitored along 8 h. The data were analyzed numerically in the time and in the frequency domain, and the results presented the ability of the technique to monitor the action of the drug, particularly Colcemid (0.4 μg/mL).

摘要

本工作提出了一种基于动态激光散斑的方案，旨在监测 MEL-RC08 癌细胞对两种不同浓度（0.2 和 0.4μg/mL）秋水仙素药物应用的反应。该方案采用前向散射方法，使用 632.8nm 的氦氖激光照射样品，同时进行对照和两种不同浓度的秋水仙素的监测，持续 8 小时。数据在时域和频域进行了数值分析，结果表明该技术能够监测药物，特别是秋水仙素（0.4μg/mL）的作用。