Yoshida M, Hara I
J Nutr Sci Vitaminol (Tokyo). 1985 Feb;31(1):69-76. doi: 10.3177/jnsv.31.69.
Effects of chlorobenzene-induced alteration in glutathione levels on cysteine metabolism in rat liver were investigated. Male Wistar rats were intraperitoneally injected with chlorobenzene (0.2, 0.5, 1.0 or 2.0 mmol/kg body weight). Both hepatic glutathione and cysteine levels were dose-dependently decreased by the chlorobenzene 6 h after the injection. However, at 24 h, the glutathione in the rats with chlorobenzene increased significantly as compared to that in the rats without chlorobenzene. Concomitant to the elevation in glutathione levels, hepatic glutathione synthesis activities were increased by the chlorobenzene by 68-111%. On the other hand, no significant difference between the rats with and without chlorobenzene was observed as regards cysteine levels at 24 h. Hepatic glutamate, glycine, methionine and serine levels were unaltered but hepatic taurine levels were significantly decreased by the chlorobenzene at both 6 and 24 h. Chlorobenzene administration had no effect on hepatic cystathionine synthase and cystathionase activities. These results indicate that a transient loss of hepatic glutathione, caused by the administration of chlorobenzene, resulted in an acceleration of glutathione synthesis and an increase of cysteine demand in the liver.
研究了氯苯诱导的谷胱甘肽水平变化对大鼠肝脏半胱氨酸代谢的影响。雄性Wistar大鼠腹腔注射氯苯（0.2、0.5、1.0或2.0 mmol/kg体重）。注射后6小时，氯苯使肝脏谷胱甘肽和半胱氨酸水平呈剂量依赖性降低。然而，在24小时时，与未注射氯苯的大鼠相比，注射氯苯的大鼠体内谷胱甘肽显著增加。伴随着谷胱甘肽水平的升高，氯苯使肝脏谷胱甘肽合成活性提高了68 - 111%。另一方面，在24小时时，注射氯苯和未注射氯苯的大鼠之间半胱氨酸水平未观察到显著差异。肝脏谷氨酸、甘氨酸、蛋氨酸和丝氨酸水平未改变，但在6小时和24小时时，氯苯均使肝脏牛磺酸水平显著降低。给予氯苯对肝脏胱硫醚合酶和胱硫醚酶活性无影响。这些结果表明，给予氯苯导致肝脏谷胱甘肽短暂缺失，从而加速了谷胱甘肽合成并增加了肝脏对半胱氨酸的需求。
